A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family

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dc.contributor.authorVarsani, Arvind
dc.contributor.authorShepherd, Dionne N
dc.contributor.authorDent, Kyle
dc.contributor.authorMonjane, Aderito L
dc.contributor.authorRybicki, Edward P
dc.contributor.authorMartin, Darren P
dc.date.accessioned2016-07-27T13:35:34Z
dc.date.available2016-07-27T13:35:34Z
dc.date.issued2009
dc.date.updated2016-07-12T17:00:32Z
dc.description.abstractBackground: We have characterised a new highly divergent geminivirus species, Eragrostis curvula streak virus (ECSV), found infecting a hardy perennial South African wild grass. ECSV represents a new genus-level geminivirus lineage, and has a mixture of features normally associated with other specific geminivirus genera. Results: Whereas the ECSV genome is predicted to express a replication associated protein (Rep) from an unspliced complementary strand transcript that is most similar to those of begomoviruses, curtoviruses and topocuviruses, its Rep also contains what is apparently a canonical retinoblastoma related protein interaction motif such as that found in mastreviruses. Similarly, while ECSV has the same unusual TAAGATTCC virion strand replication origin nonanucleotide found in another recently described divergent geminivirus, Beet curly top Iran virus (BCTIV), the rest of the transcription and replication origin is structurally more similar to those found in begomoviruses and curtoviruses than it is to those found in BCTIV and mastreviruses. ECSV also has what might be a homologue of the begomovirus transcription activator protein gene found in begomoviruses, a mastrevirus-like coat protein gene and two intergenic regions. Conclusion: Although it superficially resembles a chimaera of geminiviruses from different genera, the ECSV genome is not obviously recombinant, implying that the features it shares with other geminiviruses are those that were probably present within the last common ancestor of these viruses. In addition to inferring how the ancestral geminivirus genome may have looked, we use the discovery of ECSV to refine various hypotheses regarding the recombinant origins of the major geminivirus lineages.en_ZA
dc.identifierhttp://dx.doi.org/10.1186/1743-422X-6-36
dc.identifier.apacitationVarsani, A., Shepherd, D. N., Dent, K., Monjane, A. L., Rybicki, E. P., & Martin, D. P. (2009). A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family. <i>Virology Journal</i>, http://hdl.handle.net/11427/20903en_ZA
dc.identifier.chicagocitationVarsani, Arvind, Dionne N Shepherd, Kyle Dent, Aderito L Monjane, Edward P Rybicki, and Darren P Martin "A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family." <i>Virology Journal</i> (2009) http://hdl.handle.net/11427/20903en_ZA
dc.identifier.citationVarsani, A., Shepherd, D. N., Dent, K., Monjane, A. L., Rybicki, E. P., & Martin, D. P. (2009). A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family. Virology Journal, 6(1), 1.en_ZA
dc.identifier.issn1743-422Xen_ZA
dc.identifier.ris TY - Journal Article AU - Varsani, Arvind AU - Shepherd, Dionne N AU - Dent, Kyle AU - Monjane, Aderito L AU - Rybicki, Edward P AU - Martin, Darren P AB - Background: We have characterised a new highly divergent geminivirus species, Eragrostis curvula streak virus (ECSV), found infecting a hardy perennial South African wild grass. ECSV represents a new genus-level geminivirus lineage, and has a mixture of features normally associated with other specific geminivirus genera. Results: Whereas the ECSV genome is predicted to express a replication associated protein (Rep) from an unspliced complementary strand transcript that is most similar to those of begomoviruses, curtoviruses and topocuviruses, its Rep also contains what is apparently a canonical retinoblastoma related protein interaction motif such as that found in mastreviruses. Similarly, while ECSV has the same unusual TAAGATTCC virion strand replication origin nonanucleotide found in another recently described divergent geminivirus, Beet curly top Iran virus (BCTIV), the rest of the transcription and replication origin is structurally more similar to those found in begomoviruses and curtoviruses than it is to those found in BCTIV and mastreviruses. ECSV also has what might be a homologue of the begomovirus transcription activator protein gene found in begomoviruses, a mastrevirus-like coat protein gene and two intergenic regions. Conclusion: Although it superficially resembles a chimaera of geminiviruses from different genera, the ECSV genome is not obviously recombinant, implying that the features it shares with other geminiviruses are those that were probably present within the last common ancestor of these viruses. In addition to inferring how the ancestral geminivirus genome may have looked, we use the discovery of ECSV to refine various hypotheses regarding the recombinant origins of the major geminivirus lineages. DA - 2009 DB - OpenUCT DP - University of Cape Town J1 - Virology Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 SM - 1743-422X T1 - A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family TI - A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family UR - http://hdl.handle.net/11427/20903 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20903
dc.identifier.vancouvercitationVarsani A, Shepherd DN, Dent K, Monjane AL, Rybicki EP, Martin DP. A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family. Virology Journal. 2009; http://hdl.handle.net/11427/20903.en_ZA
dc.languageengen_ZA
dc.publisherBioMed Centralen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_ZA
dc.sourceVirology Journalen_ZA
dc.source.urihttps://virologyj.biomedcentral.com/
dc.titleA highly divergent South African geminivirus species illuminates the ancient evolutionary history of this familyen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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