Glutamine synthetase in Bacteroides fragilis

 

Show simple item record

dc.contributor.advisor Abratt, Valerie Rose en_ZA
dc.contributor.author Tumba, Nancy en_ZA
dc.date.accessioned 2015-11-08T04:59:11Z
dc.date.available 2015-11-08T04:59:11Z
dc.date.issued 2007 en_ZA
dc.identifier.citation Tumba, N. 2007. Glutamine synthetase in Bacteroides fragilis. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/14720
dc.description Includes bibliographical references (p. 78-90). en_ZA
dc.description.abstract Bactereroides fragilis is a gram-negative, non spore-forming, obligate anaerobe of the human intestinal microbiota. It is, however, an opportunistic pathogen and has been ranked as the most prevalent isolate in cases of anaerobic septicaemia. Similar to most bacteria, ammonium is assimilated in B. fragilis through the action of glutamine synthetase (GS). Glutamine is vital to nitrogen metabolism as it serves as a precursor to many secondary metabolites. GS enzymes are, therefore, vital to the growth of the organism and many prokaryotes are known to possess two or more isoforms of the enzyme. In addition, GS expression and regulation is usually tightly regulated in concert with the availability of nitrogen. Previous studies have identified a single GSIII encoding gene (glnN) in B. fragilis. In this dissertation, an additional ORF coding for a putative GSI enzyme in B. fragilis was identified, isolated and functionally characterized. A putative regulatory protein was also identified and its functional contribution to nitrogen metabolism was determined, in order to extend our understanding of nitrogen assimilation in B. fragilis. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Molecular and Cell Biology en_ZA
dc.title Glutamine synthetase in Bacteroides fragilis en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image


Files in this item

This item appears in the following Collection(s)

Show simple item record