Browsing by Subject "Sulfate"

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    Identification of population dynamics in sulfate-reducing consortia on exposure to sulphate
    (Elsevier, 2006) Icgen, B; Harrison, S T L
    The microbial population structure and function of a mixed culture of sulfate-reducing bacteria (SRB) maintained in anaerobic continuous bioreactors were tracked before and after a major perturbation, which involved the addition of sulfate to the influent of a bioreactor when operated at steady state at 35 °C, pH 7.8 and a 2.5 day residence time with feed stream containing 10 and 15 kg m−3 sulfate as terminal electron acceptor and 19.6 and 29.4 kg m−3 ethanol as carbon source and electron donor, respectively. The population structure determined by fluorescence in situ hybridization (FISH), by using 16S rRNA-targeted oligonucleotide probes, was linked to the functional performance of the SRB in the reactor. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes revealed that a high concentration of sulfate was toxic for Desulfobacterium and Desulfobulbus. On the other hand, the Desulfococcus group was found to be the most dominant group of SRB in the feed stream containing 15 kg m−3 sulfate as terminal electron acceptor and 29.4 kg m−3 ethanol as carbon source and electron donor.