Browsing by Author "Reid, Graham Charlton"

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    Studies on the regulation of extracellular collagenase production by vibrio alginolyticus
    (1981) Reid, Graham Charlton; Woods, David R; Robb, Frank T
    Vibrio alginolyticus synthesized extracellular collagenase in a highly aerated peptone medium at the late-exponential and early-stationary phases of growth. Collagenase synthesis was subject to end-product repression and was repressed by various amino acids and ammonium ions. Glutamine caused severe repression of collagenase production. Collagenase synthesis was sensitive to catabolite repression by glucose and a number of carbon sources. Cyclic AMP, dibutyryl cyclic AMP and cyclic GMP did not relieve catabolite repression. Glucose and 2-deoxyglucose caused a severe transient repression. No intracellular preformed collagenase was detected and collagenase production ceased when induced cells were washed and resuspended in buffer.Trypsin and a-chymotrypsin had no effect on collagenase production by cells or sphaeroplasts. The inducers of collagenase production in peptone were shown to have abroad molecular weight range between 1, 000 and 60,000. The peptone inducers supported slow growth of V. alginolyticus when supplied as the sole nitrogen source in minimal medium. Digestion of the peptone inducers with purified V. alginolyticus collagenase resulted in a decrease in their inducing ability,whereas digestion with trypsin or a-chymotrypsin did not. Peptone acted as an inhibitor of collagenase.