Begomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombination

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dc.contributor.authorLefeuvre, P
dc.contributor.authorMartin, D P
dc.contributor.authorHoareau, M
dc.contributor.authorNaze, F
dc.contributor.authorDelatte, H
dc.contributor.authorThierry, M
dc.contributor.authorVarsani, A
dc.contributor.authorBecker, N
dc.contributor.authorReynaud, B
dc.contributor.authorLett, J-M
dc.date.accessioned2016-07-27T12:55:08Z
dc.date.available2016-07-27T12:55:08Z
dc.date.issued2007
dc.date.updated2016-07-12T15:29:52Z
dc.description.abstractDuring the last few decades, many virus species have emerged, often forming dynamic complexes within which viruses share common hosts and rampantly exchange genetic material through recombination. Begomovirus species complexes are common and represent serious agricultural threats. Characterization of species complex diversity has substantially contributed to our understanding of both begomovirus evolution, and the ecological and epidemiological processes involved in the emergence of new viral pathogens. To date, the only extensively studied emergent African begomovirus species complex is that responsible for cassava mosaic disease. Here we present a study of another emerging begomovirus species complex which is associated with serious disease outbreaks in bean, tobacco and tomato on the south-west Indian Ocean (SWIO) islands off the coast of Africa. On the basis of 14 new complete DNA-A sequences, we describe seven new island monopartite begomovirus species, suggesting the presence of an extraordinary diversity of begomovirus in the SWIO islands. Phylogenetic analyses of these sequences reveal a close relationship between monopartite and bipartite African begomoviruses, supporting the hypothesis that either bipartite African begomoviruses have captured B components from other bipartite viruses, or there have been multiple B-component losses amongst SWIO virus progenitors. Moreover, we present evidence that detectable recombination events amongst African, Mediterranean and SWIO begomoviruses, while substantially contributing to their diversity, have not occurred randomly throughout their genomes. We provide the first statistical support for three recombination hot-spots (V1/C3 interface, C1 centre and the entire IR) and two recombination cold-spots (the V2 and the third quarter of V1) in the genomes of begomoviruses.en_ZA
dc.identifierhttp://dx.doi.org/10.1099/vir.0.83252-0
dc.identifier.apacitationLefeuvre, P., Martin, D. P., Hoareau, M., Naze, F., Delatte, H., Thierry, M., ... Lett, J. (2007). Begomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombination. <i>Journal of General Virology</i>, http://hdl.handle.net/11427/20894en_ZA
dc.identifier.chicagocitationLefeuvre, P, D P Martin, M Hoareau, F Naze, H Delatte, M Thierry, A Varsani, N Becker, B Reynaud, and J-M Lett "Begomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombination." <i>Journal of General Virology</i> (2007) http://hdl.handle.net/11427/20894en_ZA
dc.identifier.citationLefeuvre, P., Martin, D. P., Hoareau, M., Naze, F., Delatte, H., Thierry, M., ... & Lett, J. M. (2007). Begomovirus ‘melting pot’in the south-west Indian Ocean islands: molecular diversity and evolution through recombination. Journal of General Virology, 88(12), 3458-3468.en_ZA
dc.identifier.issn0022-1317en_ZA
dc.identifier.ris TY - Journal Article AU - Lefeuvre, P AU - Martin, D P AU - Hoareau, M AU - Naze, F AU - Delatte, H AU - Thierry, M AU - Varsani, A AU - Becker, N AU - Reynaud, B AU - Lett, J-M AB - During the last few decades, many virus species have emerged, often forming dynamic complexes within which viruses share common hosts and rampantly exchange genetic material through recombination. Begomovirus species complexes are common and represent serious agricultural threats. Characterization of species complex diversity has substantially contributed to our understanding of both begomovirus evolution, and the ecological and epidemiological processes involved in the emergence of new viral pathogens. To date, the only extensively studied emergent African begomovirus species complex is that responsible for cassava mosaic disease. Here we present a study of another emerging begomovirus species complex which is associated with serious disease outbreaks in bean, tobacco and tomato on the south-west Indian Ocean (SWIO) islands off the coast of Africa. On the basis of 14 new complete DNA-A sequences, we describe seven new island monopartite begomovirus species, suggesting the presence of an extraordinary diversity of begomovirus in the SWIO islands. Phylogenetic analyses of these sequences reveal a close relationship between monopartite and bipartite African begomoviruses, supporting the hypothesis that either bipartite African begomoviruses have captured B components from other bipartite viruses, or there have been multiple B-component losses amongst SWIO virus progenitors. Moreover, we present evidence that detectable recombination events amongst African, Mediterranean and SWIO begomoviruses, while substantially contributing to their diversity, have not occurred randomly throughout their genomes. We provide the first statistical support for three recombination hot-spots (V1/C3 interface, C1 centre and the entire IR) and two recombination cold-spots (the V2 and the third quarter of V1) in the genomes of begomoviruses. DA - 2007 DB - OpenUCT DP - University of Cape Town J1 - Journal of General Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 SM - 0022-1317 T1 - Begomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombination TI - Begomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombination UR - http://hdl.handle.net/11427/20894 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20894
dc.identifier.vancouvercitationLefeuvre P, Martin DP, Hoareau M, Naze F, Delatte H, Thierry M, et al. Begomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombination. Journal of General Virology. 2007; http://hdl.handle.net/11427/20894.en_ZA
dc.language.isoeng
dc.publisherMicrobiology Societyen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceJournal of General Virologyen_ZA
dc.source.urihttp://jgv.microbiologyresearch.org/content/journal/jgv
dc.titleBegomovirus 'melting pot' in the south-west Indian Ocean islands: molecular diversity and evolution through recombinationen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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