Allergenicity of latex rubber products used in South African dental schools

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dc.contributor.authorMabe, Dikeledi O
dc.contributor.authorSingh, Tanusha S
dc.contributor.authorBello, Braimoh
dc.contributor.authorJeebhay, Mohammed F
dc.contributor.authorLopata, Andreas L
dc.contributor.authorWadee, Ahmed
dc.date.accessioned2017-03-23T07:44:30Z
dc.date.available2017-03-23T07:44:30Z
dc.date.issued2009
dc.date.updated2016-01-07T09:43:53Z
dc.description.abstractBackground: Allergens from latex products in healthcare settings have been known to trigger latex induced allergic reactions in healthcare workers (HCWs). There is a need to quantify individual latex allergens in products in order to assess the allergenicity of latex products used in health care settings, so as to minimize the risk of sensitisation to these proteins. Methods: Fourteen latex examination gloves representing six brands (powdered and non-powdered) and five dental rubber dams from five dental academic institutions were analysed for latex allergens and total protein. Total protein content was determined using the BIORAD DC protein assay kit and natural rubber allergen levels using a capture ELISA assay specific for hev b 1, hev b 3, hev b 5 and hev b 6.02. Results: Hev b 6.02 was found in higher concentrations than other NRL allergens in the products analysed. Hev b 5 content ranged from 0 to 9.2µg/g and hev b 6.02 from 0.09 to 61.5µg/g of sample. Hev b 1 levels were below the detection limit (DL) for 79% of the samples (15/19). Dental dams showed higher allergen levels (median: 80.91µg/g) in comparison to latex gloves (median: 11.34µg/g). Powdered rubber samples also showed higher allergen levels (median: 40.54µg/g) compared to non-powdered samples (median: 5.31µg/g). A statistically significant correlation was observed between total protein and total allergen (r=0.74, p<0.001) concentrations. Conclusion Natural rubber latex (NRL) allergen concentrations differ significantly by product and brand. This study has demonstrated that NRL allergens in latex containing products used in South African dental institutions are present at sufficiently high levels to pose an allergic health risk.
dc.identifier.apacitationMabe, D. O., Singh, T. S., Bello, B., Jeebhay, M. F., Lopata, A. L., & Wadee, A. (2009). Allergenicity of latex rubber products used in South African dental schools. <i>South African Medical Journal</i>, http://hdl.handle.net/11427/24080en_ZA
dc.identifier.chicagocitationMabe, Dikeledi O, Tanusha S Singh, Braimoh Bello, Mohammed F Jeebhay, Andreas L Lopata, and Ahmed Wadee "Allergenicity of latex rubber products used in South African dental schools." <i>South African Medical Journal</i> (2009) http://hdl.handle.net/11427/24080en_ZA
dc.identifier.citationMabe, D. O., Singh, T. S., Bello, B., Jeebhay, M. F., Lopata, A. L., & Wadee, A. (2009). Allergenicity of latex rubber products used in south african dental schools : Original article. South African Medical Journal, 99(9), 672-674.
dc.identifier.ris TY - Journal Article AU - Mabe, Dikeledi O AU - Singh, Tanusha S AU - Bello, Braimoh AU - Jeebhay, Mohammed F AU - Lopata, Andreas L AU - Wadee, Ahmed AB - Background: Allergens from latex products in healthcare settings have been known to trigger latex induced allergic reactions in healthcare workers (HCWs). There is a need to quantify individual latex allergens in products in order to assess the allergenicity of latex products used in health care settings, so as to minimize the risk of sensitisation to these proteins. Methods: Fourteen latex examination gloves representing six brands (powdered and non-powdered) and five dental rubber dams from five dental academic institutions were analysed for latex allergens and total protein. Total protein content was determined using the BIORAD DC protein assay kit and natural rubber allergen levels using a capture ELISA assay specific for hev b 1, hev b 3, hev b 5 and hev b 6.02. Results: Hev b 6.02 was found in higher concentrations than other NRL allergens in the products analysed. Hev b 5 content ranged from 0 to 9.2µg/g and hev b 6.02 from 0.09 to 61.5µg/g of sample. Hev b 1 levels were below the detection limit (DL) for 79% of the samples (15/19). Dental dams showed higher allergen levels (median: 80.91µg/g) in comparison to latex gloves (median: 11.34µg/g). Powdered rubber samples also showed higher allergen levels (median: 40.54µg/g) compared to non-powdered samples (median: 5.31µg/g). A statistically significant correlation was observed between total protein and total allergen (r=0.74, p<0.001) concentrations. Conclusion Natural rubber latex (NRL) allergen concentrations differ significantly by product and brand. This study has demonstrated that NRL allergens in latex containing products used in South African dental institutions are present at sufficiently high levels to pose an allergic health risk. DA - 2009 DB - OpenUCT DO - 10.7196/SAMJ.3088 DP - University of Cape Town J1 - South African Medical Journal LK - https://open.uct.ac.za PB - University of Cape Town PY - 2009 T1 - Allergenicity of latex rubber products used in South African dental schools TI - Allergenicity of latex rubber products used in South African dental schools UR - http://hdl.handle.net/11427/24080 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/24080
dc.identifier.urihttp://dx.doi.org/10.7196/SAMJ.3088
dc.identifier.vancouvercitationMabe DO, Singh TS, Bello B, Jeebhay MF, Lopata AL, Wadee A. Allergenicity of latex rubber products used in South African dental schools. South African Medical Journal. 2009; http://hdl.handle.net/11427/24080.en_ZA
dc.language.isoeng
dc.publisher.departmentDivision of Immunologyen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.sourceSouth African Medical Journal
dc.source.urihttp://www.samj.org.za/index.php/samj
dc.titleAllergenicity of latex rubber products used in South African dental schools
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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