Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus

dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.authorPickering, Marken_ZA
dc.date.accessioned2015-05-13T14:17:40Z
dc.date.available2015-05-13T14:17:40Z
dc.date.issued2005en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractA high filtration resistance has been found to hinder the dewatering of the antagonistic biocontrol yeast Crytococcus albidus. Throught literature a hypothesis was developed that the extracellular polysaccharide produced by C. albidus is the major cause of hindrance to the filtration process. Microscopic analysis of C. albidus cultures illustrated cell-bound extracellular capsule. Viscosity measurements and visual observations of precipitation of the polysaccharide with ethanol were evidence extracellular polysaccharide suspended in the surrounding media. In this study, alteration of the composition of the growth medium and various pre-filtration treatments of the yeast harvest were investigated to improve filterability. The treatments included washing the yeast to remove suspended macromolecules, exposing the yeast suspension to a high shear environment to reduce the quantity of cell-bound polysaccharide, and varying suspension temperature. Us of Saccharomyces cerevisiae (Baker's yeast) and diatomaceous earth (DAE) as filter aids were investigated as a means of further improving filterability.en_ZA
dc.identifier.apacitationPickering, M. (2005). <i>Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering. Retrieved from http://hdl.handle.net/11427/12792en_ZA
dc.identifier.chicagocitationPickering, Mark. <i>"Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2005. http://hdl.handle.net/11427/12792en_ZA
dc.identifier.citationPickering, M. 2005. Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus. University of Cape Town.en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Pickering, Mark AB - A high filtration resistance has been found to hinder the dewatering of the antagonistic biocontrol yeast Crytococcus albidus. Throught literature a hypothesis was developed that the extracellular polysaccharide produced by C. albidus is the major cause of hindrance to the filtration process. Microscopic analysis of C. albidus cultures illustrated cell-bound extracellular capsule. Viscosity measurements and visual observations of precipitation of the polysaccharide with ethanol were evidence extracellular polysaccharide suspended in the surrounding media. In this study, alteration of the composition of the growth medium and various pre-filtration treatments of the yeast harvest were investigated to improve filterability. The treatments included washing the yeast to remove suspended macromolecules, exposing the yeast suspension to a high shear environment to reduce the quantity of cell-bound polysaccharide, and varying suspension temperature. Us of Saccharomyces cerevisiae (Baker's yeast) and diatomaceous earth (DAE) as filter aids were investigated as a means of further improving filterability. DA - 2005 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2005 T1 - Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus TI - Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus UR - http://hdl.handle.net/11427/12792 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12792
dc.identifier.vancouvercitationPickering M. Studies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidus. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2005 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12792en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleStudies on growth and filtration of the antagonistic biocontrol yeast Cryptococcus albidusen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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