Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions

 

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dc.contributor.advisor Jackson, Graham Ellis en_ZA
dc.contributor.author Singh, Manooj en_ZA
dc.date.accessioned 2014-11-14T03:57:45Z
dc.date.available 2014-11-14T03:57:45Z
dc.date.issued 1998 en_ZA
dc.identifier.citation Singh, M. 1998. Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/9604
dc.description Includes bibliographical references. en_ZA
dc.description.abstract Acute and Chronic inflammations in Rheumatoid Arthritis are characterized by, among other features, changes in the metabolism of copper. Previous studies have shown that administration of exogenous copper, and the in vivo manipulation of endogenous copper may provide new ways of coping with the problem of anti-inflammatory/anti-arthritic therapies. Computer models of blood plasma have attempted to rationalize the use of copper complexes in this regard. This thesis describes the design and commissioning of an ultrafiltration apparatus to study metal interactions with blood proteins. The results are compared with equilibrium dialysis. The systems studied using this technique includes, the binding of Cu(ll) to HSA and BSA, ternary Cu(ll)-HSA-amino acid systems, as well as competitive studies of the Cu(ll)-HSA system with Mg(ll), Ca(ll) and Zn(ll). The ultrafiltrate was analysed using HPLC and atomic absorption spectroscopy. Protein preparation was found to be important and the different methods used for protein purification were compared using gel electrophoresis and atomic absorption. Binding studies of chelating agents as well as several pharmaceuticals in human blood serum were carried out and compared with computer modelling studies. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Chemistry en_ZA
dc.title Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Chemistry en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Singh, M. (1998). <i>Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Chemistry. Retrieved from http://hdl.handle.net/11427/9604 en_ZA
dc.identifier.chicagocitation Singh, Manooj. <i>"Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Chemistry, 1998. http://hdl.handle.net/11427/9604 en_ZA
dc.identifier.vancouvercitation Singh M. Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Chemistry, 1998 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9604 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Singh, Manooj AB - Acute and Chronic inflammations in Rheumatoid Arthritis are characterized by, among other features, changes in the metabolism of copper. Previous studies have shown that administration of exogenous copper, and the in vivo manipulation of endogenous copper may provide new ways of coping with the problem of anti-inflammatory/anti-arthritic therapies. Computer models of blood plasma have attempted to rationalize the use of copper complexes in this regard. This thesis describes the design and commissioning of an ultrafiltration apparatus to study metal interactions with blood proteins. The results are compared with equilibrium dialysis. The systems studied using this technique includes, the binding of Cu(ll) to HSA and BSA, ternary Cu(ll)-HSA-amino acid systems, as well as competitive studies of the Cu(ll)-HSA system with Mg(ll), Ca(ll) and Zn(ll). The ultrafiltrate was analysed using HPLC and atomic absorption spectroscopy. Protein preparation was found to be important and the different methods used for protein purification were compared using gel electrophoresis and atomic absorption. Binding studies of chelating agents as well as several pharmaceuticals in human blood serum were carried out and compared with computer modelling studies. DA - 1998 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1998 T1 - Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions TI - Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions UR - http://hdl.handle.net/11427/9604 ER - en_ZA


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