The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen

 

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dc.contributor.advisor Thomson, Jennifer Ann en_ZA
dc.contributor.author Brown, Gordon Douglas en_ZA
dc.date.accessioned 2014-11-05T17:31:27Z
dc.date.available 2014-11-05T17:31:27Z
dc.date.issued 1996 en_ZA
dc.identifier.citation Brown, G. 1996. The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/9244
dc.description Bibliography: leaves 150-183. en_ZA
dc.description.abstract As lignocellulose represents an abundant renewable resource, research is in progress to obtain a better understanding of the natural mechanisms whereby this resource is utilised. Of particular interest is the degradation of forage in the rumen and one research goal is to ultimately increase animal productivity through an improvement in lignocellulose utilisation. However, although the mechanisms behind lignocellulose utilisation are reasonably well understood, relatively little is known about the mechanisms which occur in the rumen. Thus, the aim of this thesis was to gain more insight into the mechanisms of lignocellulose utilisation which occur in the rumen. Initially this research was focused on the poorly characterised exo-acting cellulases from rumen bacteria. Preliminary enzymology studies on one cellulase from Ruminococcus flavefaciens FD-1, previously isolated in this laboratory, indicated that an exo-acting cellodextrinase, CelA, had been isolated. In this report, the enzyme was purified and biochemically characterised and was shown to be an exo-acting cellodextrinase. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Microbiology en_ZA
dc.title The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Brown, G. D. (1996). <i>The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9244 en_ZA
dc.identifier.chicagocitation Brown, Gordon Douglas. <i>"The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996. http://hdl.handle.net/11427/9244 en_ZA
dc.identifier.vancouvercitation Brown GD. The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9244 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Brown, Gordon Douglas AB - As lignocellulose represents an abundant renewable resource, research is in progress to obtain a better understanding of the natural mechanisms whereby this resource is utilised. Of particular interest is the degradation of forage in the rumen and one research goal is to ultimately increase animal productivity through an improvement in lignocellulose utilisation. However, although the mechanisms behind lignocellulose utilisation are reasonably well understood, relatively little is known about the mechanisms which occur in the rumen. Thus, the aim of this thesis was to gain more insight into the mechanisms of lignocellulose utilisation which occur in the rumen. Initially this research was focused on the poorly characterised exo-acting cellulases from rumen bacteria. Preliminary enzymology studies on one cellulase from Ruminococcus flavefaciens FD-1, previously isolated in this laboratory, indicated that an exo-acting cellodextrinase, CelA, had been isolated. In this report, the enzyme was purified and biochemically characterised and was shown to be an exo-acting cellodextrinase. DA - 1996 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 T1 - The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen TI - The analysis of an enzyme (Ce1A) and a gene system (abg) involved in the utilization of lignocellulose in the rumen UR - http://hdl.handle.net/11427/9244 ER - en_ZA


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