Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262

 

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dc.contributor.advisor Reid, Sharon J en_ZA
dc.contributor.author Collett, Helen Jeanne en_ZA
dc.date.accessioned 2014-11-05T17:31:24Z
dc.date.available 2014-11-05T17:31:24Z
dc.date.issued 1996 en_ZA
dc.identifier.citation Collett, H. 1996. Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/9243
dc.description Bibliography: leaves [168]-207. en_ZA
dc.description.abstract Metronidazole is a pro-drug which must be reduced to elicit a bactericidal effect. In the clostridia, some of the electron transport proteins that provide the source of electrons for the reductive activation of metronidazole play a key role in electron distribution, which in turn regulates the direction of carbon flow in the cell. The aim of this research project was to isolate electron transport gene(s) from the solvent-producing Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole susceptibility in the C. acetobutylicum wild type was investigated. The metronidazole resistant transconjugant of interest, referred to as mutant 3R, was shown to harbour a single insertion of the Tn925: :Tn917 transposon cointegrate within a structural gene, designated sum (susceptibility to metronidazole). en_ZA
dc.language.iso eng en_ZA
dc.subject.other Microbiology en_ZA
dc.title Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Collett, H. J. (1996). <i>Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9243 en_ZA
dc.identifier.chicagocitation Collett, Helen Jeanne. <i>"Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996. http://hdl.handle.net/11427/9243 en_ZA
dc.identifier.vancouvercitation Collett HJ. Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9243 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Collett, Helen Jeanne AB - Metronidazole is a pro-drug which must be reduced to elicit a bactericidal effect. In the clostridia, some of the electron transport proteins that provide the source of electrons for the reductive activation of metronidazole play a key role in electron distribution, which in turn regulates the direction of carbon flow in the cell. The aim of this research project was to isolate electron transport gene(s) from the solvent-producing Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole susceptibility in the C. acetobutylicum wild type was investigated. The metronidazole resistant transconjugant of interest, referred to as mutant 3R, was shown to harbour a single insertion of the Tn925: :Tn917 transposon cointegrate within a structural gene, designated sum (susceptibility to metronidazole). DA - 1996 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 T1 - Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 TI - Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 UR - http://hdl.handle.net/11427/9243 ER - en_ZA


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