The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development

 

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dc.contributor.author Shoko, Aubrey Themba en_ZA
dc.date.accessioned 2014-11-03T08:14:22Z
dc.date.available 2014-11-03T08:14:22Z
dc.date.issued 2004 en_ZA
dc.identifier.citation Shoko, A. 2004. The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/9028
dc.description Includes bibliographical references. en_ZA
dc.description.abstract Due to its unique capacity to regenerate continously, the olfactory neuroepithelium serves as an excellent model system for investigating the molecular and cellular mechanisms involved in neurogenesis. The OP27 cell line (generated by infecting embryonic mouse olfactory placodes with a retrovirus carrying the temperature-sensitive alleles of the SV 40 large T antigen) was used as an in vitro system to test the effects of fibroblast growth factor-2 (FGF-2) in directing olfactory neurogenesis. The OP27 cells proliferate under the control of the retrovirus at the permissive temperature (33°C). When shifted to the non-permissive temperature (39°C) the SV40 large T antigen is inactivated and the cells stop dividing, thereby allowing one to study the effects of growth factors on these cells. Although FGF-2 also plays an important role in regulating the proliferation of neural progenitors, the main focus in this study was its effect on neuronal differentiation. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral en_ZA
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image


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