Experimental investigations of mastrevirus molecular biology and evolution

Van der Walt, Eric

dc.contributor.advisor	Rybicki, Ed	en_ZA
dc.contributor.advisor	Martin, D P	en_ZA
dc.contributor.author	Van der Walt, Eric	en_ZA
dc.date.accessioned	2014-07-30T17:42:15Z
dc.date.available	2014-07-30T17:42:15Z
dc.date.issued	2008	en_ZA
dc.identifier.citation	Van der Walt, E. 2008. Experimental investigations of mastrevirus molecular biology and evolution. University of Cape Town.	en_ZA
dc.identifier.uri	http://hdl.handle.net/11427/4346
dc.description	Includes abstract.
dc.description	Includes bibliographical references (leaves 138-161).
dc.description.abstract	This dissertation describes three major sets of experiments, all of which involved the construction and use of various reciprocal chimaeric MSV constructs. First, chimaeric viruses were used in genetic complementation-type experiments to investigate the biological significance of interactions between the two virion-sense open reading frames (ORFs) of MSV, their products, and the rest of the genome. Six chimaeric MSV constructs were made by reciprocally exchanging the ORFs encoding movement protein (MP) and coat protein (CP) individually, and in pairs, between MSV-Kom and MSV-Set, which share just 78% overall nucleotide identity. Analysis of symptomatology and infection efficiency of chimaeras and wild-type parental viruses revealed evidence of functionally relevant specific interactions between MSV MP and CP.	en_ZA
dc.language.iso	eng	en_ZA
dc.subject.other	Cell Biology	en_ZA
dc.title	Experimental investigations of mastrevirus molecular biology and evolution	en_ZA
dc.type	Doctoral Thesis
uct.type.publication	Research	en_ZA
uct.type.resource	Thesis	en_ZA
dc.publisher.institution	University of Cape Town
dc.publisher.faculty	Faculty of Science	en_ZA
dc.publisher.department	Department of Molecular and Cell Biology	en_ZA
dc.type.qualificationlevel	Doctoral
dc.type.qualificationname	PhD	en_ZA
uct.type.filetype	Text
uct.type.filetype	Image
dc.identifier.apacitation	Van der Walt, E. (2008). <i>Experimental investigations of mastrevirus molecular biology and evolution</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4346	en_ZA
dc.identifier.chicagocitation	Van der Walt, Eric. <i>"Experimental investigations of mastrevirus molecular biology and evolution."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/4346	en_ZA
dc.identifier.vancouvercitation	Van der Walt E. Experimental investigations of mastrevirus molecular biology and evolution. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4346	en_ZA
dc.identifier.ris	TY - Thesis / Dissertation AU - Van der Walt, Eric AB - This dissertation describes three major sets of experiments, all of which involved the construction and use of various reciprocal chimaeric MSV constructs. First, chimaeric viruses were used in genetic complementation-type experiments to investigate the biological significance of interactions between the two virion-sense open reading frames (ORFs) of MSV, their products, and the rest of the genome. Six chimaeric MSV constructs were made by reciprocally exchanging the ORFs encoding movement protein (MP) and coat protein (CP) individually, and in pairs, between MSV-Kom and MSV-Set, which share just 78% overall nucleotide identity. Analysis of symptomatology and infection efficiency of chimaeras and wild-type parental viruses revealed evidence of functionally relevant specific interactions between MSV MP and CP. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Experimental investigations of mastrevirus molecular biology and evolution TI - Experimental investigations of mastrevirus molecular biology and evolution UR - http://hdl.handle.net/11427/4346 ER -	en_ZA