Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene

 

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dc.contributor.advisor Thomson, Jennifer Ann en_ZA
dc.contributor.advisor Farrant, Jill M en_ZA
dc.contributor.advisor Mundree, Sagadevan G en_ZA
dc.contributor.author Maredza, Alice T en_ZA
dc.date.accessioned 2014-07-30T17:38:57Z
dc.date.available 2014-07-30T17:38:57Z
dc.date.issued 2007 en_ZA
dc.identifier.citation Maredza, A. 2007. Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/4292
dc.description Includes abstract.
dc.description Includes bibliographical references (leaves 170-209).
dc.description.abstract The Xerophyta viscosa aldose reductase cDNA (XvAld1) was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern blot analysis and DNA sequencing. The results revealed more than one copy of the gene with a complex banding pattern that was partially resolved by sequencing. The sequencing of PCRamplified genomic clones showed that the gene is organised into nine exons and eight introns spanning ~2.9 kb. The observed nucleotide differences between the sequenced clones could reflect polymorphisms between different copies of the gene. An 870-bp clone of the 5′ untranslated region, matching the 5′ leader sequence on the XvAld1 cDNA was analysed for cis-acting response elements. Many of the sequence motifs matched those for hormonal regulation, organ specific expression, dehydration, high or low temperature responses, light and phytochrome responsiveness, wounding, as well as G-box, CAAT and TATA-boxes. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Maredza, A. T. (2007). <i>Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4292 en_ZA
dc.identifier.chicagocitation Maredza, Alice T. <i>"Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2007. http://hdl.handle.net/11427/4292 en_ZA
dc.identifier.vancouvercitation Maredza AT. Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2007 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4292 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Maredza, Alice T AB - The Xerophyta viscosa aldose reductase cDNA (XvAld1) was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern blot analysis and DNA sequencing. The results revealed more than one copy of the gene with a complex banding pattern that was partially resolved by sequencing. The sequencing of PCRamplified genomic clones showed that the gene is organised into nine exons and eight introns spanning ~2.9 kb. The observed nucleotide differences between the sequenced clones could reflect polymorphisms between different copies of the gene. An 870-bp clone of the 5′ untranslated region, matching the 5′ leader sequence on the XvAld1 cDNA was analysed for cis-acting response elements. Many of the sequence motifs matched those for hormonal regulation, organ specific expression, dehydration, high or low temperature responses, light and phytochrome responsiveness, wounding, as well as G-box, CAAT and TATA-boxes. DA - 2007 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 T1 - Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene TI - Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene UR - http://hdl.handle.net/11427/4292 ER - en_ZA


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