The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae

 

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dc.contributor.advisor Lindsey, George G en_ZA
dc.contributor.advisor Brandt, Wolf F en_ZA
dc.contributor.author Karreman, Robert Jan en_ZA
dc.date.accessioned 2014-07-30T17:38:36Z
dc.date.available 2014-07-30T17:38:36Z
dc.date.issued 2006 en_ZA
dc.identifier.citation Karreman, R. 2006. The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/4278
dc.description Word processed copy.
dc.description Includes bibliographical references (leaves 117-132).
dc.description.abstract Since 1990, the yeast Saccharomyces cerevisiae small heat shock protein Hsp12p, has continuously appeared in data associated with stress responses in this organism. Hsp12p is expressed abundantly in response to a large variety of different stresses, but for many years has eluded researchers as to its function, primarily because the viability of yeast strains lacking HSP12 are unaffected by osmotic stress and heat shock. Subsequent studies indicated that Hsp12p played a role in the adaptation of the cell wall of Saccharomyces cerevisiae to conditions of stress. However, the exact in vivo localization, specific function and mediation of function of Hsp12p had yet to be elucidated. The localization of Hsp12p was determined by fusion to the green fluorescent reporter protein, Gfp2p and a combination of epifluorescent microscopy and confocal imagery. Chemical extraction revealed that Hsp12p was present in the cell wall while fluorescent imagery was not conclusive. This fluorescent Hsp12p construct was later employed in a novel application to sense the stress status of yeast, which bears future promise for use in an industrial setting. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Karreman, R. J. (2006). <i>The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4278 en_ZA
dc.identifier.chicagocitation Karreman, Robert Jan. <i>"The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006. http://hdl.handle.net/11427/4278 en_ZA
dc.identifier.vancouvercitation Karreman RJ. The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4278 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Karreman, Robert Jan AB - Since 1990, the yeast Saccharomyces cerevisiae small heat shock protein Hsp12p, has continuously appeared in data associated with stress responses in this organism. Hsp12p is expressed abundantly in response to a large variety of different stresses, but for many years has eluded researchers as to its function, primarily because the viability of yeast strains lacking HSP12 are unaffected by osmotic stress and heat shock. Subsequent studies indicated that Hsp12p played a role in the adaptation of the cell wall of Saccharomyces cerevisiae to conditions of stress. However, the exact in vivo localization, specific function and mediation of function of Hsp12p had yet to be elucidated. The localization of Hsp12p was determined by fusion to the green fluorescent reporter protein, Gfp2p and a combination of epifluorescent microscopy and confocal imagery. Chemical extraction revealed that Hsp12p was present in the cell wall while fluorescent imagery was not conclusive. This fluorescent Hsp12p construct was later employed in a novel application to sense the stress status of yeast, which bears future promise for use in an industrial setting. DA - 2006 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 T1 - The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae TI - The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae UR - http://hdl.handle.net/11427/4278 ER - en_ZA


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