Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis

 

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dc.contributor.advisor Coyne, Vernon en_ZA
dc.contributor.advisor Meyers, Ann en_ZA
dc.contributor.author Huddy, Suzanne Margaret en_ZA
dc.date.accessioned 2014-07-30T17:38:31Z
dc.date.available 2014-07-30T17:38:31Z
dc.date.issued 2011 en_ZA
dc.identifier.citation Huddy, S. 2011. Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/4274
dc.description Includes abstract.
dc.description Includes bibliographical references.
dc.description.abstract A better understanding of how this commercially important seaweed responds at a genetic level to stresses faced in the aquaculture environment would not only be advantageous to the South African industry, but this knowledge is essential for selecting and/or engineering macroalgal strains that are either more tolerant or resistant to these stresses. This requires in vivo analysis of G. gracilis gene function and regulation in order to introduce new or improved genes into G. gracilis, and for this to be possible, a protocol for transformation of recombinant DNA into G. gracilis is required. In this study a transformation and tissue culture system for G. gracilis was developed. These tools provide the necessary groundwork for future genetic manipulation studies that are essential for improving our understanding of the role that various genes play in stress response and tolerance in G. gracilis. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Cell Biology en_ZA
dc.title Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Huddy, S. M. (2011). <i>Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4274 en_ZA
dc.identifier.chicagocitation Huddy, Suzanne Margaret. <i>"Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011. http://hdl.handle.net/11427/4274 en_ZA
dc.identifier.vancouvercitation Huddy SM. Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4274 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Huddy, Suzanne Margaret AB - A better understanding of how this commercially important seaweed responds at a genetic level to stresses faced in the aquaculture environment would not only be advantageous to the South African industry, but this knowledge is essential for selecting and/or engineering macroalgal strains that are either more tolerant or resistant to these stresses. This requires in vivo analysis of G. gracilis gene function and regulation in order to introduce new or improved genes into G. gracilis, and for this to be possible, a protocol for transformation of recombinant DNA into G. gracilis is required. In this study a transformation and tissue culture system for G. gracilis was developed. These tools provide the necessary groundwork for future genetic manipulation studies that are essential for improving our understanding of the role that various genes play in stress response and tolerance in G. gracilis. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis TI - Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis UR - http://hdl.handle.net/11427/4274 ER - en_ZA


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