Cloning and characterisation of a bZIP transcription factor from a resurrection grass, Eragrostis nindensis

Master Thesis

2004

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University of Cape Town

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Abstract
The G-box is a plant DNA cis-acting element involved in the regulation of gene expression in response to a range of environmental signals including anaerobiosis, dehydration and light as well as by abscisic acid (ABA). Basic leucine zipper (bZIP) transcription factors have been shown to specifically bind and activate transcription from G-boxes in a dimerized form. A 1.5 kb cDNA for a bZIP class transcription factor, designated EnGBF1, was cloned from a desiccation-tolerant grass, Eragrostis nindensis by degenerate RT-PCR.
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Includes bibliographical references (leaves 43-56).

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