Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector

 

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dc.contributor.author van Diepen, Michiel
dc.contributor.author Chapman, Rosamund
dc.contributor.author Douglass, Nicola
dc.contributor.author Whittle, Leah
dc.contributor.author Chineka, Nicole
dc.contributor.author Galant, Shireen
dc.contributor.author Cotchobos, Christian
dc.contributor.author Suzuki, Akiko
dc.contributor.author Williamson, Anna-Lise
dc.date.accessioned 2021-10-25T11:20:17Z
dc.date.available 2021-10-25T11:20:17Z
dc.date.issued 2021-10-04
dc.identifier 10.3390/vaccines9101131
dc.identifier.citation van Diepen, M., Chapman, R., Douglass, N., Whittle, L., Chineka, N., Galant, S., Cotchobos, C. & Suzuki, A. et al. 2021. Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector. <i>Vaccines.</i> 9(10):1131. http://hdl.handle.net/11427/35287 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/35287
dc.description.abstract Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) testis (LT) cells, Madin–Darby bovine kidney (MDBK) cells or in eggs. Growth in eggs is a laborious process, the use of primary cells has the potential to introduce pathogens and MDBK cells are known to harbor bovine viral diarrhea virus (BVDV). In this study, data is presented to show the growth of an attenuated LSDV strain in baby hamster kidney (BHK-21) cells. Subsequently, a recombinant LSDV vaccine was generated in BHK-21 cells. Partial growth was also observed in rabbit kidney cells (RK13), but only when the vaccinia virus host range gene K1L was expressed. Despite the limited growth, the expression of K1L was enough to serve as a positive selection marker for the generation of recombinant LSDV vaccines in RK13 cells. The simplification of generating (recombinant) LSDV vaccines shown here should increase the interest for this platform for future livestock vaccine development and, with BHK-21 cells approved for current good manufacturing practice, this can be expanded to human vaccines as well. en_US
dc.language.iso en en_US
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en_US
dc.source Vaccines en_US
dc.source.uri https://www.mdpi.com/journal/vaccines
dc.title Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector en_US
dc.type Journal Article en_US
dc.date.updated 2021-10-22T13:55:44Z
dc.publisher.faculty Faculty of Health Sciences en_US
dc.publisher.department Department of Pathology en_US
dc.source.journalvolume 9 en_US
dc.source.journalissue 10 en_US
dc.source.pagination 1131 en_US
dc.identifier.apacitation van Diepen, M., Chapman, R., Douglass, N., Whittle, L., Chineka, N., Galant, S., ... Williamson, A. (2021). Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector. <i>Vaccines</i>, 9(10), 1131. http://hdl.handle.net/11427/35287 en_ZA
dc.identifier.chicagocitation van Diepen, Michiel, Rosamund Chapman, Nicola Douglass, Leah Whittle, Nicole Chineka, Shireen Galant, Christian Cotchobos, Akiko Suzuki, and Anna-Lise Williamson "Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector." <i>Vaccines</i> 9, 10. (2021): 1131. http://hdl.handle.net/11427/35287 en_ZA
dc.identifier.vancouvercitation van Diepen M, Chapman R, Douglass N, Whittle L, Chineka N, Galant S, et al. Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector. Vaccines. 2021;9(10):1131. http://hdl.handle.net/11427/35287. en_ZA
dc.identifier.ris TY - Journal Article AU - van Diepen, Michiel AU - Chapman, Rosamund AU - Douglass, Nicola AU - Whittle, Leah AU - Chineka, Nicole AU - Galant, Shireen AU - Cotchobos, Christian AU - Suzuki, Akiko AU - Williamson, Anna-Lise AB - Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) testis (LT) cells, Madin–Darby bovine kidney (MDBK) cells or in eggs. Growth in eggs is a laborious process, the use of primary cells has the potential to introduce pathogens and MDBK cells are known to harbor bovine viral diarrhea virus (BVDV). In this study, data is presented to show the growth of an attenuated LSDV strain in baby hamster kidney (BHK-21) cells. Subsequently, a recombinant LSDV vaccine was generated in BHK-21 cells. Partial growth was also observed in rabbit kidney cells (RK13), but only when the vaccinia virus host range gene K1L was expressed. Despite the limited growth, the expression of K1L was enough to serve as a positive selection marker for the generation of recombinant LSDV vaccines in RK13 cells. The simplification of generating (recombinant) LSDV vaccines shown here should increase the interest for this platform for future livestock vaccine development and, with BHK-21 cells approved for current good manufacturing practice, this can be expanded to human vaccines as well. DA - 2021-10-04 DB - OpenUCT DP - University of Cape Town IS - 10 J1 - Vaccines LK - https://open.uct.ac.za PY - 2021 T1 - Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector TI - Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector UR - http://hdl.handle.net/11427/35287 ER - en_ZA


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