Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species

Doctoral Thesis

2000

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http://hdl.handle.net/11427/3464
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thesis_hsf_2000_siziba_k_b.pdf (15.94 MB)
Authors
Siziba, Kwanele Bennett
Supervisors
Meissner, Peter
Corrigall, Anne
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University of Cape Town

Department
Department of Medicine
Faculty
Faculty of Health Sciences
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Abstract
This work involved the characterisation of protoporphyrinogen oxidase (PPO), the penultimate enzyme in haem biosynthesis, from Bacillus subtilis, Myxococcus xanthus, and human. A defect in human PPO causes variegate porphyria, an autosomal dominant disorder characterised by skin photosensitivity and propensity towards acute neurovisceral crises. At the beginning of this project little information was available on the kinetic and biophysical properties of isolated PPOs due largely to difficulties associated with their purification from natural sources.
Description

Bibliography: leaves 163-203.

Keywords
Medicine

Reference:

Siziba, K. 2000. Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species. University of Cape Town.

Collections
PhD / Doctoral