Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species

Siziba, Kwanele Bennett

dc.contributor.advisor	Meissner, Peter	en_ZA
dc.contributor.advisor	Corrigall, Anne	en_ZA
dc.contributor.author	Siziba, Kwanele Bennett	en_ZA
dc.date.accessioned	2014-07-29T09:10:30Z
dc.date.available	2014-07-29T09:10:30Z
dc.date.issued	2000	en_ZA
dc.identifier.citation	Siziba, K. 2000. Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species. University of Cape Town.	en_ZA
dc.identifier.uri	http://hdl.handle.net/11427/3464
dc.description	Bibliography: leaves 163-203.
dc.description.abstract	This work involved the characterisation of protoporphyrinogen oxidase (PPO), the penultimate enzyme in haem biosynthesis, from Bacillus subtilis, Myxococcus xanthus, and human. A defect in human PPO causes variegate porphyria, an autosomal dominant disorder characterised by skin photosensitivity and propensity towards acute neurovisceral crises. At the beginning of this project little information was available on the kinetic and biophysical properties of isolated PPOs due largely to difficulties associated with their purification from natural sources.	en_ZA
dc.language.iso	eng	en_ZA
dc.subject.other	Medicine	en_ZA
dc.title	Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species	en_ZA
dc.type	Doctoral Thesis
uct.type.publication	Research	en_ZA
uct.type.resource	Thesis	en_ZA
dc.publisher.institution	University of Cape Town
dc.publisher.faculty	Faculty of Health Sciences	en_ZA
dc.publisher.department	Department of Medicine	en_ZA
dc.type.qualificationlevel	Doctoral
dc.type.qualificationname	PhD	en_ZA
uct.type.filetype	Text
uct.type.filetype	Image
dc.identifier.apacitation	Siziba, K. B. (2000). <i>Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/3464	en_ZA
dc.identifier.chicagocitation	Siziba, Kwanele Bennett. <i>"Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2000. http://hdl.handle.net/11427/3464	en_ZA
dc.identifier.vancouvercitation	Siziba KB. Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2000 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3464	en_ZA
dc.identifier.ris	TY - Thesis / Dissertation AU - Siziba, Kwanele Bennett AB - This work involved the characterisation of protoporphyrinogen oxidase (PPO), the penultimate enzyme in haem biosynthesis, from Bacillus subtilis, Myxococcus xanthus, and human. A defect in human PPO causes variegate porphyria, an autosomal dominant disorder characterised by skin photosensitivity and propensity towards acute neurovisceral crises. At the beginning of this project little information was available on the kinetic and biophysical properties of isolated PPOs due largely to difficulties associated with their purification from natural sources. DA - 2000 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2000 T1 - Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species TI - Expression, purification and characterisation of protoporphyrinogen oxidases from diverse species UR - http://hdl.handle.net/11427/3464 ER -	en_ZA