The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools

 

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dc.contributor.advisor Dheda, Keertan en_ZA
dc.contributor.advisor Khati, Makobetsa en_ZA
dc.contributor.advisor Gresh, Lionel en_ZA
dc.contributor.author Maserumule, Matsopiane Charlotte en_ZA
dc.date.accessioned 2014-07-29T09:07:14Z
dc.date.available 2014-07-29T09:07:14Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Maserumule, M. 2013. The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/3425
dc.description Includes abstract.
dc.description Includes bibliographical references.
dc.description.abstract Lack of point-of-care (PoC) diagnostic tools for TB hinders control of the disease, particularly in resource-limited, high HIV and TB prevalence countries. Therefore, there is a need for simple, rapid, accurate, and affordable PoC diagnostics to detect active TB early enough for opportune intervention. To develop TB detection probes that will constitute such diagnostics, our research group recently isolated DNA aptamers that bind to a putative marker for active TB; the ESAT-6.CFP-10 heterodimer. Aptamers are highly specific artificial mimics of antibodies that have shown great prospects in diagnostic applications. The aim of this study was to characterise the anti-ESAT-6.CFP-10 aptamers, and to optimise them into more specific and affordable detection probes for the development of potential PoC TB diagnostic tools. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Medicine en_ZA
dc.title The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Medicine en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Maserumule, M. C. (2013). <i>The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/3425 en_ZA
dc.identifier.chicagocitation Maserumule, Matsopiane Charlotte. <i>"The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013. http://hdl.handle.net/11427/3425 en_ZA
dc.identifier.vancouvercitation Maserumule MC. The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3425 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Maserumule, Matsopiane Charlotte AB - Lack of point-of-care (PoC) diagnostic tools for TB hinders control of the disease, particularly in resource-limited, high HIV and TB prevalence countries. Therefore, there is a need for simple, rapid, accurate, and affordable PoC diagnostics to detect active TB early enough for opportune intervention. To develop TB detection probes that will constitute such diagnostics, our research group recently isolated DNA aptamers that bind to a putative marker for active TB; the ESAT-6.CFP-10 heterodimer. Aptamers are highly specific artificial mimics of antibodies that have shown great prospects in diagnostic applications. The aim of this study was to characterise the anti-ESAT-6.CFP-10 aptamers, and to optimise them into more specific and affordable detection probes for the development of potential PoC TB diagnostic tools. DA - 2013 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools TI - The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools UR - http://hdl.handle.net/11427/3425 ER - en_ZA


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