The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools
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| dc.contributor.advisor | Dheda, Keertan | en_ZA |
| dc.contributor.advisor | Khati, Makobetsa | en_ZA |
| dc.contributor.advisor | Gresh, Lionel | en_ZA |
| dc.contributor.author |
Maserumule, Matsopiane Charlotte
|
en_ZA |
| dc.date.accessioned | 2014-07-29T09:07:14Z | |
| dc.date.available | 2014-07-29T09:07:14Z | |
| dc.date.issued | 2013 | en_ZA |
| dc.identifier.citation | Maserumule, M. 2013. The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools. University of Cape Town. | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/3425 | |
| dc.description | Includes abstract. | |
| dc.description | Includes bibliographical references. | |
| dc.description.abstract | Lack of point-of-care (PoC) diagnostic tools for TB hinders control of the disease, particularly in resource-limited, high HIV and TB prevalence countries. Therefore, there is a need for simple, rapid, accurate, and affordable PoC diagnostics to detect active TB early enough for opportune intervention. To develop TB detection probes that will constitute such diagnostics, our research group recently isolated DNA aptamers that bind to a putative marker for active TB; the ESAT-6.CFP-10 heterodimer. Aptamers are highly specific artificial mimics of antibodies that have shown great prospects in diagnostic applications. The aim of this study was to characterise the anti-ESAT-6.CFP-10 aptamers, and to optimise them into more specific and affordable detection probes for the development of potential PoC TB diagnostic tools. | en_ZA |
| dc.language.iso | eng | en_ZA |
| dc.subject.other | Medicine | en_ZA |
| dc.title | The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools | en_ZA |
| dc.type | Master Thesis | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Thesis | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.publisher.faculty | Faculty of Health Sciences | en_ZA |
| dc.publisher.department | Department of Medicine | en_ZA |
| dc.type.qualificationlevel | Masters | |
| dc.type.qualificationname | MSc | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| dc.identifier.apacitation | Maserumule, M. C. (2013). <i>The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/3425 | en_ZA |
| dc.identifier.chicagocitation | Maserumule, Matsopiane Charlotte. <i>"The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013. http://hdl.handle.net/11427/3425 | en_ZA |
| dc.identifier.vancouvercitation | Maserumule MC. The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3425 | en_ZA |
| dc.identifier.ris | TY - Thesis / Dissertation AU - Maserumule, Matsopiane Charlotte AB - Lack of point-of-care (PoC) diagnostic tools for TB hinders control of the disease, particularly in resource-limited, high HIV and TB prevalence countries. Therefore, there is a need for simple, rapid, accurate, and affordable PoC diagnostics to detect active TB early enough for opportune intervention. To develop TB detection probes that will constitute such diagnostics, our research group recently isolated DNA aptamers that bind to a putative marker for active TB; the ESAT-6.CFP-10 heterodimer. Aptamers are highly specific artificial mimics of antibodies that have shown great prospects in diagnostic applications. The aim of this study was to characterise the anti-ESAT-6.CFP-10 aptamers, and to optimise them into more specific and affordable detection probes for the development of potential PoC TB diagnostic tools. DA - 2013 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools TI - The development of aptamer-based probes for the detection of TB antigens ESAT-6.CFP-10 potential TB diagnostic tools UR - http://hdl.handle.net/11427/3425 ER - | en_ZA |
