Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection

 

Show simple item record

dc.contributor.advisor Brombacher, Frank en_ZA
dc.contributor.author Einhorn, Andrew en_ZA
dc.date.accessioned 2014-07-29T09:03:20Z
dc.date.available 2014-07-29T09:03:20Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Einhorn, A. 2013. Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/3380
dc.description Includes abstract.
dc.description Includes bibliographical references.
dc.description.abstract This thesis is composed of two parts. The first is an investigation of the role of B-effector cells in the course of murine Leishmania Major (L. major). The second is a bioinformatic analysis of microarray slides of activated CD4 T-cells from L. major infected BALB/c and C57BL/6 mice in order to investigate the genetic determinants of their divergent disease phenotypes. Overall this thesis is an investigation of the role of B-and T-lymphocytes during the course of L. major infection in mice. B-cells are not traditionally thought to play a role in the pathogenesis of Leishmania major infection. It is well documented that T-helper 1(Th1) and T-helper 2 (Th2) responses lead to resistance and susceptiblity to L. major respectively. Recent studies have now shown that B-cells are capable to producing key T-cell differentiating cytokines such as IL-4 and IFN-γ. More specifically, two new B-cell populations have been identified: B effector 1 (Be1) and B effector 2 (Be2) cells, which produce IFN-γ and IL-4 respectively. Using mice lacking the IL-4Rα on B-cells, and thus incapble of producing Be2 cells, we investigate the contribution of Be2 cells to BALB/c susceptibility. We confirm that the delection of the IL-4Rα on B-cells renders the previously susceptible BALB/c mice resistant to L. major strain LV39 and further confirm the phenotype using the more the virulent IL81 strain. We demonstrate that mice lacking Be2 cells exhibit a dimished Th2 response and an enhanced Th1 response, suggesting that B-cells, in addition to their role in antibody production, may also play a role in shaping T-helper responses in vivo. In the case of L.major, this provides evidence for a novel link between B cells and the cellular immune response. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Medicine en_ZA
dc.title Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Medicine en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Einhorn, A. (2013). <i>Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/3380 en_ZA
dc.identifier.chicagocitation Einhorn, Andrew. <i>"Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013. http://hdl.handle.net/11427/3380 en_ZA
dc.identifier.vancouvercitation Einhorn A. Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2013 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/3380 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Einhorn, Andrew AB - This thesis is composed of two parts. The first is an investigation of the role of B-effector cells in the course of murine Leishmania Major (L. major). The second is a bioinformatic analysis of microarray slides of activated CD4 T-cells from L. major infected BALB/c and C57BL/6 mice in order to investigate the genetic determinants of their divergent disease phenotypes. Overall this thesis is an investigation of the role of B-and T-lymphocytes during the course of L. major infection in mice. B-cells are not traditionally thought to play a role in the pathogenesis of Leishmania major infection. It is well documented that T-helper 1(Th1) and T-helper 2 (Th2) responses lead to resistance and susceptiblity to L. major respectively. Recent studies have now shown that B-cells are capable to producing key T-cell differentiating cytokines such as IL-4 and IFN-γ. More specifically, two new B-cell populations have been identified: B effector 1 (Be1) and B effector 2 (Be2) cells, which produce IFN-γ and IL-4 respectively. Using mice lacking the IL-4Rα on B-cells, and thus incapble of producing Be2 cells, we investigate the contribution of Be2 cells to BALB/c susceptibility. We confirm that the delection of the IL-4Rα on B-cells renders the previously susceptible BALB/c mice resistant to L. major strain LV39 and further confirm the phenotype using the more the virulent IL81 strain. We demonstrate that mice lacking Be2 cells exhibit a dimished Th2 response and an enhanced Th1 response, suggesting that B-cells, in addition to their role in antibody production, may also play a role in shaping T-helper responses in vivo. In the case of L.major, this provides evidence for a novel link between B cells and the cellular immune response. DA - 2013 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection TI - Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection UR - http://hdl.handle.net/11427/3380 ER - en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record