Diagnostic performance of glomerular PLA2R and THSD7A antibodies in biopsy confirmed primary membranous nephropathy in South Africans

 

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dc.contributor.author Lwezaula, Bingileki F
dc.contributor.author Ameh, Oluwatoyin I
dc.contributor.author Ekrikpo, Udeme E
dc.contributor.author Botha, Francois C
dc.contributor.author Okpechi-Samuel, Ugochi S
dc.contributor.author Wearne, Nicola
dc.contributor.author Ronco, Pierre
dc.contributor.author Bello, Aminu K
dc.contributor.author Okpechi, Ikechi G
dc.date.accessioned 2021-06-29T20:04:34Z
dc.date.available 2021-06-29T20:04:34Z
dc.date.issued 2021-01-07
dc.identifier.uri https://doi.org/10.1186/s12882-020-02216-7
dc.identifier.uri http://hdl.handle.net/11427/33420
dc.description.abstract Background: Serum and tissue-based tests using phospholipase A2 receptor 1 (PLA2R) and thrombospondin type-1 domain containing 7A (THSD7A) are established immune biomarkers for the diagnosis of primary membranous nephropathy (PMN). This study assessed the diagnostic performance of these biomarkers in the diagnosis of PMN in South Africans. Methods This was a cross-sectional analysis from a single centre in Cape Town, South Africa. Relevant biodata was collected from all patients. Histology, including slides for PLA2R and THSD7A were processed and assessed by typical microscopic and immunohistochemical features. Biopsy tissues of patients with membranous lupus nephritis (LN-V) and diabetic nephropathy (DN) were used as controls. The diagnostic accuracy for diagnosis of PMN using positive PLA2R and THSD7A were evaluated. Results Of the 88 patients included, 41 had PMN with a mean age of 44.5 ± 17.5 years and 61.0% were female. Histologically, PLA2R and THSD7A were only positive in the PMN group (51.2% and 4.9%, respectively) but negative in both control groups. The sensitivity of PLA2R and THSD7A for identifying PMN was 51.2% and 4.9%, respectively. The sensitivity of both tests together was 53.7% while the specificity and positive predictive values (PPV) for any of the tests (alone or in combination) was 100%. There was no difference in the sensitivity and specificity when using PLA2R alone compared to combining the two tests (p=0.32). Conclusion Glomerular staining of PLA2R and THSD7A could have potential diagnostic values in South Africans. This has implications on how immunotherapies can be initiated and used in these settings.
dc.source BMC Nephrology
dc.source BMC Nephrology
dc.source.uri https://bmcnephrol.biomedcentral.com/
dc.subject Africans
dc.subject Primary membranous nephropathy
dc.subject Lupus nephritis
dc.subject PLA2R
dc.subject THSD7A
dc.title Diagnostic performance of glomerular PLA2R and THSD7A antibodies in biopsy confirmed primary membranous nephropathy in South Africans
dc.date.updated 2021-01-10T04:12:35Z
dc.language.rfc3066 en
dc.rights.holder The Author(s)
dc.source.journalvolume 22
dc.source.journalissue 1
dc.source.pagination 15
dc.identifier.ris TY - AU - Lwezaula, Bingileki F AU - Ameh, Oluwatoyin I AU - Ekrikpo, Udeme E AU - Botha, Francois C AU - Okpechi-Samuel, Ugochi S AU - Wearne, Nicola AU - Ronco, Pierre AU - Bello, Aminu K AU - Okpechi, Ikechi G AB - Background: Serum and tissue-based tests using phospholipase A2 receptor 1 (PLA2R) and thrombospondin type-1 domain containing 7A (THSD7A) are established immune biomarkers for the diagnosis of primary membranous nephropathy (PMN). This study assessed the diagnostic performance of these biomarkers in the diagnosis of PMN in South Africans. Methods This was a cross-sectional analysis from a single centre in Cape Town, South Africa. Relevant biodata was collected from all patients. Histology, including slides for PLA2R and THSD7A were processed and assessed by typical microscopic and immunohistochemical features. Biopsy tissues of patients with membranous lupus nephritis (LN-V) and diabetic nephropathy (DN) were used as controls. The diagnostic accuracy for diagnosis of PMN using positive PLA2R and THSD7A were evaluated. Results Of the 88 patients included, 41 had PMN with a mean age of 44.5 ± 17.5 years and 61.0% were female. Histologically, PLA2R and THSD7A were only positive in the PMN group (51.2% and 4.9%, respectively) but negative in both control groups. The sensitivity of PLA2R and THSD7A for identifying PMN was 51.2% and 4.9%, respectively. The sensitivity of both tests together was 53.7% while the specificity and positive predictive values (PPV) for any of the tests (alone or in combination) was 100%. There was no difference in the sensitivity and specificity when using PLA2R alone compared to combining the two tests (p=0.32). Conclusion Glomerular staining of PLA2R and THSD7A could have potential diagnostic values in South Africans. This has implications on how immunotherapies can be initiated and used in these settings. DA - 2021-01-07 DB - OpenUCT DP - University of Cape Town IS - 1 J1 - BMC Nephrology KW - Africans KW - Primary membranous nephropathy KW - Lupus nephritis KW - PLA2R KW - THSD7A LK - https://open.uct.ac.za PY - 2021 T1 - Diagnostic performance of glomerular PLA2R and THSD7A antibodies in biopsy confirmed primary membranous nephropathy in South Africans TI - Diagnostic performance of glomerular PLA2R and THSD7A antibodies in biopsy confirmed primary membranous nephropathy in South Africans UR - http://hdl.handle.net/11427/33420 ER - en_ZA


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