Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America

 

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dc.contributor.author Fontenele, Rafaela S
dc.contributor.author Bhaskara, Amulya
dc.contributor.author Cobb, Ilaria N
dc.contributor.author Majure, Lucas C
dc.contributor.author Salywon, Andrew M
dc.contributor.author Avalos-Calleros, Jesús A
dc.contributor.author Argüello-Astorga, Gerardo R
dc.contributor.author Schmidlin, Kara
dc.contributor.author Roumagnac, Philippe
dc.contributor.author Ribeiro, Simone G
dc.contributor.author Kraberger, Simona
dc.contributor.author Martin, Darren P
dc.contributor.author Lefeuvre, Pierre
dc.contributor.author Varsani, Arvind
dc.date.accessioned 2021-06-23T12:43:57Z
dc.date.available 2021-06-23T12:43:57Z
dc.date.issued 2021-04-30
dc.identifier doi: 10.3390/v13050810
dc.identifier.citation Fontenele, R.S., Bhaskara, A., Cobb, I.N., Majure, L.C., Salywon, A.M., Avalos-Calleros, J.A., Argüello-Astorga, G.R. & Schmidlin, K. et al. 2021. Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America. <i>Viruses.</i> 13(5) http://hdl.handle.net/11427/33384 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/33384
dc.description.abstract Geminiviruses are a group of plant-infecting viruses with single-stranded DNA genomes. Within this family, viruses in the genus Begomovirus are known to have a worldwide distribution causing a range of severe diseases in a multitude of dicotyledonous plant species. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and their ssDNA genomes can be either monopartite or bipartite. As part of a viral survey, various plants including those in the families Alliaceae, Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Malvaceae, Oleaceae and Solanaceae were sampled and screened for begomoviruses using both a high-throughput sequencing and a begomovirus-specific primer pair approach. Based on the sequences derived using these approaches, the full-length genome of various begomoviruses were amplified from plants using abutting primers. Squash leaf curl virus (SLCV) and watermelon chlorotic stunt virus (WCSV) were identified in Cactaceae (n = 25), Solanaceae (n = 7), Cucurbitaceae (n = 2) and Lamiaceae (n = 1) samples. WCSV is an Old World bipartite begomovirus that has only recently been discovered infecting watermelons in the Americas. Our discovery of WCSV in the USA is the first indication that it has reached this country and indicates that this virus might be widespread throughout North America. Phylogenetic analysis suggests WCSV was introduced to the New World twice. The detection of begomoviruses in cactus plants suggests possible spillover events from agricultural areas into native vegetation. Since WCSV and SLCV have previously been found in mixed infections, pseudo-recombination infection experiments were conducted. We demonstrate that WCSV DNA-B is successfully trans-replicated by SLCV DNA-A despite very low degree of similarity between the replication-associated iterative sequences present in their common region, an essential feature for binding of the replication associated protein. This study highlights the importance of viral surveys for the detection of spillover events into native vegetation, but also suggests the need for more surveillance of WCSV in the USA, as this virus is a serious threat to watermelon cultivation in the Middle East.
dc.source Viruses
dc.source.uri https://www.mdpi.com/journal/viruses
dc.title Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America
dc.type Journal Article
dc.date.updated 2021-05-24T15:10:56Z
dc.source.journalvolume 13
dc.source.journalissue 5
dc.identifier.apacitation Fontenele, R. S., Bhaskara, A., Cobb, I. N., Majure, L. C., Salywon, A. M., Avalos-Calleros, J. A., ... Varsani, A. (2021). Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America. <i>Viruses</i>, 13(5), http://hdl.handle.net/11427/33384 en_ZA
dc.identifier.chicagocitation Fontenele, Rafaela S, Amulya Bhaskara, Ilaria N Cobb, Lucas C Majure, Andrew M Salywon, Jesús A Avalos-Calleros, Gerardo R Argüello-Astorga, et al "Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America." <i>Viruses</i> 13, 5. (2021) http://hdl.handle.net/11427/33384 en_ZA
dc.identifier.vancouvercitation Fontenele RS, Bhaskara A, Cobb IN, Majure LC, Salywon AM, Avalos-Calleros JA, et al. Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America. Viruses. 2021;13(5) http://hdl.handle.net/11427/33384. en_ZA
dc.identifier.ris TY - Journal Article AU - Fontenele, Rafaela S AU - Bhaskara, Amulya AU - Cobb, Ilaria N AU - Majure, Lucas C AU - Salywon, Andrew M AU - Avalos-Calleros, Jesús A AU - Argüello-Astorga, Gerardo R AU - Schmidlin, Kara AU - Roumagnac, Philippe AU - Ribeiro, Simone G AU - Kraberger, Simona AU - Martin, Darren P AU - Lefeuvre, Pierre AU - Varsani, Arvind AB - Geminiviruses are a group of plant-infecting viruses with single-stranded DNA genomes. Within this family, viruses in the genus Begomovirus are known to have a worldwide distribution causing a range of severe diseases in a multitude of dicotyledonous plant species. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and their ssDNA genomes can be either monopartite or bipartite. As part of a viral survey, various plants including those in the families Alliaceae, Amaranthaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Malvaceae, Oleaceae and Solanaceae were sampled and screened for begomoviruses using both a high-throughput sequencing and a begomovirus-specific primer pair approach. Based on the sequences derived using these approaches, the full-length genome of various begomoviruses were amplified from plants using abutting primers. Squash leaf curl virus (SLCV) and watermelon chlorotic stunt virus (WCSV) were identified in Cactaceae (n = 25), Solanaceae (n = 7), Cucurbitaceae (n = 2) and Lamiaceae (n = 1) samples. WCSV is an Old World bipartite begomovirus that has only recently been discovered infecting watermelons in the Americas. Our discovery of WCSV in the USA is the first indication that it has reached this country and indicates that this virus might be widespread throughout North America. Phylogenetic analysis suggests WCSV was introduced to the New World twice. The detection of begomoviruses in cactus plants suggests possible spillover events from agricultural areas into native vegetation. Since WCSV and SLCV have previously been found in mixed infections, pseudo-recombination infection experiments were conducted. We demonstrate that WCSV DNA-B is successfully trans-replicated by SLCV DNA-A despite very low degree of similarity between the replication-associated iterative sequences present in their common region, an essential feature for binding of the replication associated protein. This study highlights the importance of viral surveys for the detection of spillover events into native vegetation, but also suggests the need for more surveillance of WCSV in the USA, as this virus is a serious threat to watermelon cultivation in the Middle East. DA - 2021-04-30 DB - OpenUCT DP - University of Cape Town IS - 5 J1 - Viruses LK - https://open.uct.ac.za PY - 2021 T1 - Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America TI - Identification of the Begomoviruses Squash Leaf Curl Virus and Watermelon Chlorotic Stunt Virus in Various Plant Samples in North America UR - http://hdl.handle.net/11427/33384 ER - en_ZA


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