Morphological investigations into the development of the mammalian corneal endothelium using the mouse model

 

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dc.contributor.advisor Kidson, Sue en_ZA
dc.contributor.author Mgwebi, Thandiswa en_ZA
dc.date.accessioned 2014-07-28T18:17:30Z
dc.date.available 2014-07-28T18:17:30Z
dc.date.issued 2004 en_ZA
dc.identifier.citation Mgwebi, T. 2004. Morphological investigations into the development of the mammalian corneal endothelium using the mouse model. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/3268
dc.description Includes bibliographical references (leaves 83-89).
dc.description.abstract The corneal endothelium (CE), a mesenchyme-derived tissue, is a monolayer of squamous cells on the inner corneal surface. In Foxc1-1• mice, the CE fails to form. The understanding of the cause of this defect has implications for the study of human eye disorders that are related to FOXC1 mutations. To understand the basis of CE defects in Foxc1-1- mice, an analysis of normal CE development was performed using scanning electron microscopy. Results showed that in normal mice the transformation from mesenchyme to endothelium was initiated at embryonic day (E) 12.5 and was characterised by a change from stellate to cobblestone shape and the formation of junctions. In FoxcN- mice, the process was initiated but a cobblestone shape not attained. The expression of adherens (N-cadherin) and tight junction (Z0-1) proteins was investigated by immunoflouresence microscopy. In the normal embryo, the expression of N-cadherin was initially in cytoplasmic vesicles and later at the cell membranes. ZO-l was first detected at the cell peripheries at E13.5. In Foxct-I- mice, N-cadherin peripheral bands failed to form. ZO-l was not expressed. These results suggest that the failure to form a monolayered CE in Foxc1 mice is due to incomplete mesenchyme-endothelial conversion. Junction formation was further investigated in vitro. N-cadherin was cytoplasmic in pre-confluent cells and at cell edges in confluent cells. ZO-l was not detected. These results suggest that in vitro, these cells are either unable to form tight junctions or the culture medium does not contain the appropriate signalling molecules. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Medicine en_ZA
dc.title Morphological investigations into the development of the mammalian corneal endothelium using the mouse model en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Human Biology en_ZA
dc.type.qualificationlevel Doctoral en_ZA
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image


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