Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit

 

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dc.contributor.advisor Heathfield, Laura J en_ZA
dc.contributor.author Nel, Lorraine en_ZA
dc.date.accessioned 2018-05-14T12:26:14Z
dc.date.available 2018-05-14T12:26:14Z
dc.date.issued 2018 en_ZA
dc.identifier.citation Nel, L. 2018. Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/28054
dc.description.abstract DNA profiling is routinely used in the forensic setting to identify individuals during criminal and medico-legal investigations. Its principle is based on the molecular analysis of DNA to produce a string of alpha-numeric characters which can be matched to a known reference sample. The use of allele frequencies from the background population aids the statistical interpretation of a match and can be used to calculate the random match probability. In South Africa, allele frequency data for the background population is currently limited, which can hinder the discriminatory value of DNA evidence, particularly when only a partial profile is obtained. Therefore, the aim of this study was to generate DNA allele frequency data for four South African population groups using the QIAGEN Investigator® 24PLEX GO! Kit, which has six markers for which data does not yet exist for the South African population. Full forensic DNA profiles were generated from 655 unrelated individuals from four population groups in South Africa: Black African (n = 172), Coloured (n = 195), Indian/Asian (n = 88) and White (n = 200). A 98% first time success rate was observed using the direct PCR approach. Allele frequencies were significantly different between all four population groups at three markers (D8S1179, D2S1338 and D2S441) after a Bonferroni correction (ρ < 0.001) and sixteen novel alleles were observed. Two genetic anomalies were observed, namely triallelic patterns at the TPOX marker (n = 9) and a null allele at amelogenin (n = 1). While the sample size for the Indian/Asian population group was limited in this study, the data generated here nevertheless prospects to contribute towards the data currently published for South Africa. This, in turn, will allow for more DNA markers to be analysed during forensic casework in South Africa, as the data for its statistical interpretation is now available. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Biomedical Forensic Science en_ZA
dc.title Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of Forensic Medicine and Toxicology en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MPhil en_ZA
uct.type.filetype Text
uct.type.filetype Image


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