Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles

 

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dc.contributor.author Pang, Liping
dc.contributor.author Farkas, Kata
dc.contributor.author Bennett, Grant
dc.contributor.author Varsani, Arvind
dc.contributor.author Easingwood, Richard
dc.contributor.author Tilley, Richard
dc.contributor.author Nowostawska, Urszula
dc.contributor.author Lin, Susan
dc.date.accessioned 2018-03-26T11:54:59Z
dc.date.available 2018-03-26T11:54:59Z
dc.date.issued 2014
dc.identifier http://dx.doi.org/10.1016/j.watres.2014.05.055
dc.identifier.citation Pang, L., Farkas, K., Bennett, G., Varsani, A., Easingwood, R., Tilley, R., . . . Lin, S. (2014). Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles. Water Research, 62, 167-179. doi:10.1016/j.watres.2014.05.055
dc.identifier.uri http://hdl.handle.net/11427/27719
dc.description.abstract Abstract Rotavirus (RoV) and adenovirus (AdV) are important viral pathogens for the risk analysis of drinking water. Despite this, little is known about their retention and transport behaviors in porous media due to a lack of representative surrogates. We developed RoV and AdV surrogates by covalently coupling 70-nm sized silica nanoparticles with specific proteins and a DNA marker for sensitive detection. Filtration experiments using beach sand columns demonstrated the similarity of the surrogates' concentrations, filtration efficiencies and attachment kinetics to those of the target viruses. The surrogates showed the same magnitude of concentration reduction as the viruses. Conversely, MS2 phage (a traditional virus model) over-predicted concentrations of AdV and RoV by 1- and 2-orders of magnitude respectively. The surrogates remained stable in size, surface charge and DNA concentration for at least one year. They can be easily and rapidly detected down to a single particle. Preliminary tests suggest that they were readily detectable in a number of environmental waters and treated effluent. With up-scaling validation in pilot trials, the surrogates developed here could be a cost-effective new tool for studying virus retention and transport in porous media. Examples include assessing filter efficacy in water and wastewater treatment, tracking virus migration in groundwater after effluent land disposal, and establishing safe setback distances for groundwater protection.
dc.source Water Research
dc.source.uri http://www.journals.elsevier.com/water-research
dc.subject.other Virus surrogates
dc.subject.other Nanoparticles
dc.subject.other Sand filtration
dc.subject.other Groundwater
dc.subject.other DNA-labeling
dc.subject.other Porous media
dc.title Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles
dc.type Journal Article
dc.date.updated 2016-01-20T13:00:13Z
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of Medical Biochemistry en_ZA
uct.type.filetype Text
uct.type.filetype Image


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