In this study, novel dimers of the biologically active phenolic compound totarol were synthesized using the phenol oxidase enzyme laccase, obtained from Trametes pusbescens, in organic solvent medium. Two dimeric products, linked either by carbon-carbon or by carbon-oxygen bonds, were isolated and characterized. The effect of changes in various parameters such as solvent, temperature, pH and buffer concentration on the conversion of totarol by laccase was investigated. The nature of the organic solvent, in particular, was found to affect the nature and the ratio of the products obtained.
Reference:
Ncanana, S., Baratto, L., Roncaglia, L., Riva, S., & Burton, S. G. (2007). Laccase‐mediated oxidation of totarol. Advanced Synthesis & Catalysis, 349(8‐9), 1507-1513.
Ncanana, S., & Baratto, L. (2007). Laccase-mediated oxidation of totarol. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering. Retrieved from http://hdl.handle.net/11427/24155
Ncanana, Sandile, and Lara Baratto. "Laccase-mediated oxidation of totarol." Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2007. http://hdl.handle.net/11427/24155
Ncanana S, Baratto L. Laccase-mediated oxidation of totarol. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 2007 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/24155