Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus

 

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dc.contributor.advisor Woods, David R en_ZA
dc.contributor.advisor Robb, Frank T en_ZA
dc.contributor.author Hare, Patricia en_ZA
dc.date.accessioned 2016-10-25T13:36:56Z
dc.date.available 2016-10-25T13:36:56Z
dc.date.issued 1982 en_ZA
dc.identifier.citation Hare, P. 1982. Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/22301
dc.description Bibliography: pages 143-167. en_ZA
dc.description.abstract The production of an extracellular collagenase and serine proteases by Vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37°C and by lack of oxygen. V. alginolyticus had identical growth rates at 30 and 37°C. Aeration did not affect the growth rate of stationary phase cells when the exoproteases were being produced. Macromolecular synthesis in stationary phase cells was not affected by temperature. The regulation of exoprotease production by temperature and oxygen is specific and has implications regarding the ecology of V. alginolyticus. The synthesis of a 100 000 molecular weight protein was induced in V. alginolyticus by either raising the temperature from 30 to 37°C, a lack of oxygen or (NH₄)₂SO₄. Histidine stimulated synthesis of a 52 000 molecular weight protein. The possibility that these proteins have a regulatory role in exoenzyme synthesis is discussed. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Microbiology en_ZA
dc.title Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Hare, P. (1982). <i>Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22301 en_ZA
dc.identifier.chicagocitation Hare, Patricia. <i>"Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982. http://hdl.handle.net/11427/22301 en_ZA
dc.identifier.vancouvercitation Hare P. Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22301 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Hare, Patricia AB - The production of an extracellular collagenase and serine proteases by Vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37°C and by lack of oxygen. V. alginolyticus had identical growth rates at 30 and 37°C. Aeration did not affect the growth rate of stationary phase cells when the exoproteases were being produced. Macromolecular synthesis in stationary phase cells was not affected by temperature. The regulation of exoprotease production by temperature and oxygen is specific and has implications regarding the ecology of V. alginolyticus. The synthesis of a 100 000 molecular weight protein was induced in V. alginolyticus by either raising the temperature from 30 to 37°C, a lack of oxygen or (NH₄)₂SO₄. Histidine stimulated synthesis of a 52 000 molecular weight protein. The possibility that these proteins have a regulatory role in exoenzyme synthesis is discussed. DA - 1982 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1982 T1 - Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus TI - Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus UR - http://hdl.handle.net/11427/22301 ER - en_ZA


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