Fermentation studies on Clostridium acetabutylicum

 

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dc.contributor.advisor Woods, David R en_ZA
dc.contributor.advisor Jones, David T en_ZA
dc.contributor.author Van der Westhuizen, André en_ZA
dc.date.accessioned 2016-10-10T14:57:01Z
dc.date.available 2016-10-10T14:57:01Z
dc.date.issued 1982 en_ZA
dc.identifier.citation Van der Westhuizen, A. 1982. Fermentation studies on Clostridium acetabutylicum. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/22111
dc.description Bibliography: pages 147-162. en_ZA
dc.description.abstract The initial aim of this work was to develop a laboratory system for the study of the ABE fermentation under laboratory conditions. The development of defined and simple laboratory inoculation and build-up procedures for the ABE process was investigated. A defined spore preparation in distilled water gave solvent yields comparable to the yields obtained in the commercial ABE process. A laboratory inoculation procedure was developed which avoided the lengthy culture build-up procedures presently utilised. An investigation into solvent production by Clostridium acetobutylicum in clostridial basal medium (CBM) , reinforced clostridial medium (RCM) , Leung and Robson media was undertaken with the aim of developing a partially defined laboratory medium which produced solvent yields comparable to the molasses fermentation medium (MFM). The solvent yields obtained in the partially defined laboratory media were substantially lower than those obtained in MFM. It became apparent that the initial aim of trying to identify and manipulate a few key factors to give better solvent yields would not be easily attained. Both the solvent levels and the overall pattern of cell development were markedly different in the various laboratory systems. In view of these differences, a more detailed investigation of the growth patterns, morphological and physiological changes were undertaken. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Microbiology en_ZA
dc.title Fermentation studies on Clostridium acetabutylicum en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Van der Westhuizen, A. (1982). <i>Fermentation studies on Clostridium acetabutylicum</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22111 en_ZA
dc.identifier.chicagocitation Van der Westhuizen, André. <i>"Fermentation studies on Clostridium acetabutylicum."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982. http://hdl.handle.net/11427/22111 en_ZA
dc.identifier.vancouvercitation Van der Westhuizen A. Fermentation studies on Clostridium acetabutylicum. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22111 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Van der Westhuizen, André AB - The initial aim of this work was to develop a laboratory system for the study of the ABE fermentation under laboratory conditions. The development of defined and simple laboratory inoculation and build-up procedures for the ABE process was investigated. A defined spore preparation in distilled water gave solvent yields comparable to the yields obtained in the commercial ABE process. A laboratory inoculation procedure was developed which avoided the lengthy culture build-up procedures presently utilised. An investigation into solvent production by Clostridium acetobutylicum in clostridial basal medium (CBM) , reinforced clostridial medium (RCM) , Leung and Robson media was undertaken with the aim of developing a partially defined laboratory medium which produced solvent yields comparable to the molasses fermentation medium (MFM). The solvent yields obtained in the partially defined laboratory media were substantially lower than those obtained in MFM. It became apparent that the initial aim of trying to identify and manipulate a few key factors to give better solvent yields would not be easily attained. Both the solvent levels and the overall pattern of cell development were markedly different in the various laboratory systems. In view of these differences, a more detailed investigation of the growth patterns, morphological and physiological changes were undertaken. DA - 1982 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1982 T1 - Fermentation studies on Clostridium acetabutylicum TI - Fermentation studies on Clostridium acetabutylicum UR - http://hdl.handle.net/11427/22111 ER - en_ZA


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