The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum

 

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dc.contributor.advisor Woods, David R en_ZA
dc.contributor.author Ziniades, Catherine en_ZA
dc.date.accessioned 2016-10-03T04:06:55Z
dc.date.available 2016-10-03T04:06:55Z
dc.date.issued 1990 en_ZA
dc.identifier.citation Ziniades, C. 1990. The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/22047
dc.description Bibliography: pages 163-169. en_ZA
dc.description.abstract The objective of this work was to produce γ-linolenic acid (γLA) using a fungus in submerged fermentation. Selection work was aimed at identifying a fungal strain capable of yielding a high level of γLA in an industrial fermentation. Thirty-nine fungal strains were screened under shake flask conditions. The major criteria used in evaluating these strains were, the yield of γLA per unit volume (g/l) and γLA as a percentage of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial importance such as strain handling and the fatty acid profile were also considered. Eleven fungi in the order Phycomycetes were identified after initial screening. From these fungi, a strain of Choanephora cucurbitarum was found to give superior γLA yields. c. cucurbitarum produced γLA yields of 331mg/l and 674mg/l in shake flask and laboratory fermenters respectively. This strain had other industrially beneficial qualities such as good sporulation, a good biomass of 22, 5g/l and a relatively high yield of γLA of 2,99g/100g dry matter. Subsequently a Zygorhynchus heterogamus strain was found to give similar yields of γLA to c. cucurbitarum. z. heterogamus also had a high γLA : linoleic acid ratio which aids the purification of γLA . This is the first known report of a high level of γLA in the genus Zygorhynchus. The industrial development of γLA production by Zygorhynchus is not reported. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Oils and fats - Microbiology en_ZA
dc.title The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Ziniades, C. (1990). <i>The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/22047 en_ZA
dc.identifier.chicagocitation Ziniades, Catherine. <i>"The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1990. http://hdl.handle.net/11427/22047 en_ZA
dc.identifier.vancouvercitation Ziniades C. The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1990 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/22047 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ziniades, Catherine AB - The objective of this work was to produce γ-linolenic acid (γLA) using a fungus in submerged fermentation. Selection work was aimed at identifying a fungal strain capable of yielding a high level of γLA in an industrial fermentation. Thirty-nine fungal strains were screened under shake flask conditions. The major criteria used in evaluating these strains were, the yield of γLA per unit volume (g/l) and γLA as a percentage of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial importance such as strain handling and the fatty acid profile were also considered. Eleven fungi in the order Phycomycetes were identified after initial screening. From these fungi, a strain of Choanephora cucurbitarum was found to give superior γLA yields. c. cucurbitarum produced γLA yields of 331mg/l and 674mg/l in shake flask and laboratory fermenters respectively. This strain had other industrially beneficial qualities such as good sporulation, a good biomass of 22, 5g/l and a relatively high yield of γLA of 2,99g/100g dry matter. Subsequently a Zygorhynchus heterogamus strain was found to give similar yields of γLA to c. cucurbitarum. z. heterogamus also had a high γLA : linoleic acid ratio which aids the purification of γLA . This is the first known report of a high level of γLA in the genus Zygorhynchus. The industrial development of γLA production by Zygorhynchus is not reported. DA - 1990 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1990 T1 - The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum TI - The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum UR - http://hdl.handle.net/11427/22047 ER - en_ZA


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