Gene cloning studies in two nocardioform bacteria

 

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dc.contributor.advisor Woods, David R en_ZA
dc.contributor.author Hill, Russell en_ZA
dc.date.accessioned 2016-09-25T16:46:55Z
dc.date.available 2016-09-25T16:46:55Z
dc.date.issued 1988 en_ZA
dc.identifier.citation Hill, R. 1988. Gene cloning studies in two nocardioform bacteria. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21896
dc.description Bibliography: pages 147-177. en_ZA
dc.description.abstract Nocardioforms are Gram-positive, aerobic actinomycetes and are a metabolically diverse group which produce antibiotics, useful enzymes, are important in the biotransformation of organic compounds and the decomposition of organic wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of actinomycete genomic libraries. Cloning of DNA into the unique Bg1II endonuclease site of pLR591 inactivated the lethal EcoRI gene derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived from pIJ702 was shown to be functional in Streptomyces lividans enabling selection of recombinant pLR591 plasmids containing foreign DNA in S. lividans. The vector pLR591 therefore functions as a positive selection Streptomyces-E. coli shuttle vector facilitating construction of actinomycete genomic libraries in E. coli and subsequent transfer of recombinant plasmids into S. lividans. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Actinomycetales en_ZA
dc.subject.other Bacterial genetics en_ZA
dc.title Gene cloning studies in two nocardioform bacteria en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral en_ZA
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image


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