Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization

 

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dc.contributor.advisor Woods, David R en_ZA
dc.contributor.advisor Rawlings , Douglas E en_ZA
dc.contributor.author Meyers, Paul Robert en_ZA
dc.date.accessioned 2016-09-14T13:00:46Z
dc.date.available 2016-09-14T13:00:46Z
dc.date.issued 1993 en_ZA
dc.identifier.citation Meyers, P. 1993. Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21775
dc.description Bibliography: p. 217-233. en_ZA
dc.description.abstract A cyanide-degrading, Gram-positive, aerobic, endospore-forming bacterium was isolated from a cyanide wastewater dam by an enrichment technique and was identified as a strain of Bacillus pumilus. The bacterium was routinely cultured in Oxoid nutrient broth and rapidly degraded 100 mg/1 of free cyanide in the absence of added inorganic and organic substances. The ability to degrade cyanide was linked to the growth phase and was not exhibited before late-exponential/ early-stationary phase. Cyanide-degrading activity could not be induced in early exponential phase by the addition of cyanide or acetonitrile to 20 mg/1. Production of the cyanide-degrading activity required at least 0.01 mg Mn2+ /1 in the growth medium (lower concentrations prevented the development of strong cyanide-degrading activity and also resulted in poor growth of the organism). No induction of cyanide-degrading activity occurred when Mn2+ ions were added to late-exponential-phase cells (or a cell-free extract from these cells) which had been grown with a low endogenous concentration of Mn2+. However, Mn2+ ions could be added to cultures growing in low-Mn2+ broth as late as the mid-exponential phase of growth with no apparent reduction of the cyanidedegrading activity exhibited in stationary phase. Culturing the organism in Difeo nutrient broth resulted in poor growth and very low levels of cyanide-degrading activity; addition of Mn2+ to this medium did not significantly increase the levels of activity. Production of the cyanide-degrading activity required de novo transcription and translation. Cyanide-degrading activity was located intracellularly and cell-free extracts rapidly degraded cyanide (0.27 ± 0.08 μmole cyanide/min/mg protein at 30 °C in pH 7.4 phosphate buffer). Eight strains of cyanide-utilizing fluorescent pseudomonads were also isolated from activated sewage sludge by an enrichment technique and tentatively identified as strains of P. fluorescens and P. putida. These isolates could not degrade cyanide rapidly. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Molecular and Cell Biology en_ZA
dc.title Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Meyers, P. R. (1993). <i>Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21775 en_ZA
dc.identifier.chicagocitation Meyers, Paul Robert. <i>"Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1993. http://hdl.handle.net/11427/21775 en_ZA
dc.identifier.vancouvercitation Meyers PR. Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1993 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21775 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Meyers, Paul Robert AB - A cyanide-degrading, Gram-positive, aerobic, endospore-forming bacterium was isolated from a cyanide wastewater dam by an enrichment technique and was identified as a strain of Bacillus pumilus. The bacterium was routinely cultured in Oxoid nutrient broth and rapidly degraded 100 mg/1 of free cyanide in the absence of added inorganic and organic substances. The ability to degrade cyanide was linked to the growth phase and was not exhibited before late-exponential/ early-stationary phase. Cyanide-degrading activity could not be induced in early exponential phase by the addition of cyanide or acetonitrile to 20 mg/1. Production of the cyanide-degrading activity required at least 0.01 mg Mn2+ /1 in the growth medium (lower concentrations prevented the development of strong cyanide-degrading activity and also resulted in poor growth of the organism). No induction of cyanide-degrading activity occurred when Mn2+ ions were added to late-exponential-phase cells (or a cell-free extract from these cells) which had been grown with a low endogenous concentration of Mn2+. However, Mn2+ ions could be added to cultures growing in low-Mn2+ broth as late as the mid-exponential phase of growth with no apparent reduction of the cyanidedegrading activity exhibited in stationary phase. Culturing the organism in Difeo nutrient broth resulted in poor growth and very low levels of cyanide-degrading activity; addition of Mn2+ to this medium did not significantly increase the levels of activity. Production of the cyanide-degrading activity required de novo transcription and translation. Cyanide-degrading activity was located intracellularly and cell-free extracts rapidly degraded cyanide (0.27 ± 0.08 μmole cyanide/min/mg protein at 30 °C in pH 7.4 phosphate buffer). Eight strains of cyanide-utilizing fluorescent pseudomonads were also isolated from activated sewage sludge by an enrichment technique and tentatively identified as strains of P. fluorescens and P. putida. These isolates could not degrade cyanide rapidly. DA - 1993 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1993 T1 - Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization TI - Cyanide degradation by Bacillus pumilus C1 : cellular and molecular characterization UR - http://hdl.handle.net/11427/21775 ER - en_ZA


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