Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli

 

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dc.contributor.advisor Rawlings, Douglas E en_ZA
dc.contributor.author Brown, Lynn Dryden en_ZA
dc.date.accessioned 2016-09-06T14:44:36Z
dc.date.available 2016-09-06T14:44:36Z
dc.date.issued 1993 en_ZA
dc.identifier.citation Brown, L. 1993. Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21697
dc.description.abstract An atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the transformants/transductants to grow on succinate as the sole non-fermentable carbon source was used to select mutants with a functional F₁F₀ ATPsynthase. Many F₁ -complementing plasmids and cosmids were isolated from the four E. coli F₁ unc point mutants screened. No plasmids or cosmids which complemented an E. coli fΔunc strain or any of the three E. coli F₀ mutants screened, were isolated. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Microbiology en_ZA
dc.title Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli en_ZA
dc.type Thesis en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel PhD en_ZA
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image


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