A modified pH drift assay for inorganic carbon accumulation and external carbonic anhydrase activity in microalgae

 

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dc.contributor.author van Hille, R
dc.contributor.author Fagan, M
dc.contributor.author Bromfield, L
dc.contributor.author Pott, R
dc.date.accessioned 2016-08-25T10:35:24Z
dc.date.available 2016-08-25T10:35:24Z
dc.date.issued 2014
dc.identifier http://dx.doi.org/10.1007/s10811-013-0076-6
dc.identifier.citation van Hille, R., Fagan, M., Bromfield, L., & Pott, R. (2014). A modified pH drift assay for inorganic carbon accumulation and external carbonic anhydrase activity in microalgae. Journal of applied phycology, 26(1), 377-385. en_ZA
dc.identifier.issn 0921-8971 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21541
dc.description.abstract Threat of global warming due to carbon dioxide (CO2) emissions has stimulated research into carbon sequestration and emissions reduction technologies. Alkaline scrubbing allows CO2 to be captured as bicarbonate, which can be photochemically fixed by microalgae. The carbon concentrating mechanism (CCM), of which external carbonic anhydrase is a key component, allows organisms to utilise this bicarbonate. In order to select a suitable strain for this application, a screening tool is required. The current method for determining carbonic anhydrase activity, the Wilbur and Anderson assay, was found to be unsuitable as a screening tool as the associated error was unacceptably large and tests on whole cells were inconclusive. This paper presents the development of a new, whole cell assay to measure inorganic carbon uptake and external carbonic anhydrase activity, based on classical pH drift experiments. Spirulina platensis was successfully used to develop a correlation between the specific carbon uptake (C) and the specific pH change (dpH). The relationship is described by the following: C[mmol C (g dry algae)−1 h−1] = 0.064 × (dpH). Inhibitor and salt dissociation tests validated the activity and presence of external carbonic anhydrase and allowed correlation between the Wilbur and Anderson assay and the new whole cell assay. Screening tests were conducted on S. platensis, Scenedesmus sp., Chlorella vulgaris and Dunaliella salina that were found to have carbon uptake rates of 5.76, 5.86, 3.86 and 2.15 mmol C (g dry algae)−1 h−1, respectively. These results corresponded to the species' known bicarbonate utilisation abilities and validated the use of the assay as a screening tool. en_ZA
dc.language eng en_ZA
dc.publisher Springer en_ZA
dc.source Journal of Applied Phycology en_ZA
dc.source.uri http://link.springer.com/journal/10811
dc.subject.other Aqueous chemistry
dc.subject.other Carbon concentrating mechanism
dc.subject.other Screening test
dc.title A modified pH drift assay for inorganic carbon accumulation and external carbonic anhydrase activity in microalgae en_ZA
dc.type Journal Article en_ZA
dc.date.updated 2016-08-23T10:04:55Z
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
uct.type.filetype Text
uct.type.filetype Image


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