Physiological and genetic evidence for an OmpB signal transduction system in Erwinia chrysanthemi

 

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dc.contributor.advisor Qhobela, Molapo en_ZA
dc.contributor.author Crampton, Michael Craig en_ZA
dc.date.accessioned 2016-08-22T12:21:59Z
dc.date.available 2016-08-22T12:21:59Z
dc.date.issued 1996 en_ZA
dc.identifier.citation Crampton, M. 1996. Physiological and genetic evidence for an OmpB signal transduction system in Erwinia chrysanthemi. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21410
dc.description Bibliography: pages 132-155. en_ZA
dc.description.abstract In order for bacteria to survive in their environment they must continuely sense signals such as, presence of host organisms, chemical concentrations, or variationsin other physiological parameters. Many bacteria sense their environment through the use of a two component regulatory systems. These systems usually employ the use of two different proteins, a sensor protein and its cognate response regulator. Some bacteria can survive fluctuations in medium osmolarity through the use of a two component signal transduction system. In Escherichia coli and Salmonella typhimurium this two component system includes the EnvZ sensor protein and its cognate response regulator, OmpR. The two genes that code for these proteins are envZ and ompR genes respectively. The two genes together form the ompB operonrespectively. This operon regulates the expression of two outer membrane proteins, OmpF and OmpC in response to medium osmolarity in E. coli.Erwinia chrysanthemi has been found to be sensitive to desication. Proliferation of soft rot, caused by this organism, has also been associated with irrigation. E.chrysanthemi has also been observed to respond to changes in medium osmolarity. Evidence of an ompB operon was thus sought. Outer membrane proteins were isolated using sodium lauroylsarcosine. Three major outer membrane proteins were isolated, namely Ompl (37.5 kd), Omp2 (35.5 kd) and Omp3 (34.5 kd). Increase in medium osmolarity resulted in an increase in expression of Omp3, while Ompl was suppressed. This lends support to the presence of an ompB like signal transduction system in E. chrysanthemi. Growth temperature was shown to have no effect on the expression of the major OMP. Similarly, culture growth phase had no effect on major OMP expression. However, two induced OMP were present from mid log phase onwards. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Molecular and Cell Biology en_ZA
dc.title Physiological and genetic evidence for an OmpB signal transduction system in Erwinia chrysanthemi en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image


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