Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects

 

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dc.contributor.advisor Heyns, Marise en_ZA
dc.contributor.advisor Mole, Calvin Gerald en_ZA
dc.contributor.author Sanyanga, Taremekedzwa en_ZA
dc.date.accessioned 2016-08-19T13:16:53Z
dc.date.available 2016-08-19T13:16:53Z
dc.date.issued 2016 en_ZA
dc.identifier.citation Sanyanga, T. 2016. Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21372
dc.description.abstract When the death of an individual has occurred, the body of the deceased is not always discovered immediately and at times the body may be discovered after a long period of time. The consequence of discovering a body after a long period of time is that the body maybe found severely decomposed or skeletonized. As a result no viable blood, urine or tissue samples may exist that can be collected and utilized in toxicological analyses. Entomotoxicology offers a supplementary method to detect and analyse the presence of drugs post-mortem, especially in cases where viable toxicological specimens such as human tissue cannot be obtained. In South Africa and globally, standardised methodology is required to perform entomological examinations accurately, however due to the large variation of experimental set-up no such standardised methods yet exist. The main aim of the research was to analyse the effect of Amitriptyline on the development and growth rate of forensically important blow flies, and to investigate the potential in using blow fly larvae of the Western Cape, South Africa in forensic entomotoxicological analyses and future implementation. To achieve this blow fly species C. chloropyga and L. sericata were reared on homogenised pig liver containing 1000 mg/kg Amitriptyline until emergence of imago. The duration of time taken by the blow fly larvae species to reach 1 st , 2 nd , 3 rd , pupae and imago growth stages in the presence and absence of Amitriptyline was noted. The presence of drugs in larvae was investigated using high-pressure liquid chromatography coupled with dual mass spectrometry (HPLC/MS-MS). Amitriptyline was detected in all experimental larvae samples and was found to delay pupation by at least 26 hours and imago emergence by at least 72 hours. Amitriptyline however showed no distinct effect on C. chloropyga length and mass but was associated with a smaller length and mass in L. sericata compared to controls. Results indicate that entomotoxicology can be of use for qualitative analysis for the presence of Amitriptyline in forensic cases. Results also highlight the unpredictability of research using biological specimens. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Biomedical Forensic Science en_ZA
dc.title Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of Forensic Medicine and Toxicology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MPhil en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Sanyanga, T. (2016). <i>Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology. Retrieved from http://hdl.handle.net/11427/21372 en_ZA
dc.identifier.chicagocitation Sanyanga, Taremekedzwa. <i>"Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016. http://hdl.handle.net/11427/21372 en_ZA
dc.identifier.vancouvercitation Sanyanga T. Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21372 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Sanyanga, Taremekedzwa AB - When the death of an individual has occurred, the body of the deceased is not always discovered immediately and at times the body may be discovered after a long period of time. The consequence of discovering a body after a long period of time is that the body maybe found severely decomposed or skeletonized. As a result no viable blood, urine or tissue samples may exist that can be collected and utilized in toxicological analyses. Entomotoxicology offers a supplementary method to detect and analyse the presence of drugs post-mortem, especially in cases where viable toxicological specimens such as human tissue cannot be obtained. In South Africa and globally, standardised methodology is required to perform entomological examinations accurately, however due to the large variation of experimental set-up no such standardised methods yet exist. The main aim of the research was to analyse the effect of Amitriptyline on the development and growth rate of forensically important blow flies, and to investigate the potential in using blow fly larvae of the Western Cape, South Africa in forensic entomotoxicological analyses and future implementation. To achieve this blow fly species C. chloropyga and L. sericata were reared on homogenised pig liver containing 1000 mg/kg Amitriptyline until emergence of imago. The duration of time taken by the blow fly larvae species to reach 1 st , 2 nd , 3 rd , pupae and imago growth stages in the presence and absence of Amitriptyline was noted. The presence of drugs in larvae was investigated using high-pressure liquid chromatography coupled with dual mass spectrometry (HPLC/MS-MS). Amitriptyline was detected in all experimental larvae samples and was found to delay pupation by at least 26 hours and imago emergence by at least 72 hours. Amitriptyline however showed no distinct effect on C. chloropyga length and mass but was associated with a smaller length and mass in L. sericata compared to controls. Results indicate that entomotoxicology can be of use for qualitative analysis for the presence of Amitriptyline in forensic cases. Results also highlight the unpredictability of research using biological specimens. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects TI - Entomological examination of the relationship between ante-mortem and post-mortem amitriptyline concentrations in insects UR - http://hdl.handle.net/11427/21372 ER - en_ZA


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