Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation

 

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dc.contributor.author Reynders, M B
dc.contributor.author Rawlings, D E
dc.contributor.author Harrison, S T L
dc.date.accessioned 2016-08-17T08:42:14Z
dc.date.available 2016-08-17T08:42:14Z
dc.date.issued 1997
dc.identifier http://dx.doi.org/10.1023/A:1018341421122
dc.identifier.citation Reynders, M. B., Rawlings, D. E., & Harrison, S. T. L. (1997). Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation. Biotechnology letters, 19(6), 549-552. en_ZA
dc.identifier.issn 0141-5492 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21281
dc.description.abstract By monitoring cell yield and fermentation products during fed-batch and continuous growth, Pfaffia rhodozyma was shown to exhibit the Crabtree effect. In fed-batch culture at feed concentrations of 27 and 55 g glucose/l there was good agreement between the observed biomass formation and that predicted by a mass balance model. At 125 g glucose/l in the feed, biomass formation was less than predicted and fermentation products such as ethanol and acetic acid accumulated in the culture medium. In continuous culture with a feed concentration of 10 g glucose/l, the Crabtree effect became apparent at a dilution rate of 0.1 h -1 . Aerobic fermentation did not occur provided the sugar substrate was maintained at a concentration of less than 0.5 g/l. Although the cell yield coefficient was reduced from 0.5 g/g to 0.16 g/g during aerobic fermentation, the carotenoid content of the cells was unaffected. en_ZA
dc.language eng en_ZA
dc.publisher Springer en_ZA
dc.source Biotechnology Letters en_ZA
dc.source.uri http://link.springer.com/journal/10529
dc.title Demonstration of the Crabtree effect in Phaffia rhodozyma during continuous and fed-batch cultivation en_ZA
dc.type Journal Article en_ZA
dc.date.updated 2016-08-16T10:32:11Z
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
uct.type.filetype Text
uct.type.filetype Image


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