Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair

 

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dc.contributor.author Cooper, Keren
dc.contributor.author Farrant, Jill M
dc.date.accessioned 2016-08-04T14:06:44Z
dc.date.available 2016-08-04T14:06:44Z
dc.date.issued 2002
dc.identifier http://dx.doi.org/10.1093/jxb/erf028
dc.identifier.citation Cooper, K., & Farrant, J. M. (2002). Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair. Journal of Experimental Botany, 53(375), 1805-1813. en_ZA
dc.identifier.issn 0022-0957 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/21124
dc.description.abstract Craterostigma wilmsii Engl. (homoiochlorophyllous) is a resurrection species that is thought to rely primarily on the protection of cellular components during drying to survive desiccation. The time taken for this protection to be instituted is thought to preclude recovery after rapid drying. Thus the response of C. wilmsii plants to rapid dehydration was investigated. The effect of rapid drying on sucrose accumulation was determined and the cellular ultrastructure was investigated during natural (slow) and rapid dehydration and on subsequent rehydration. The dependence of naturally and rapidly dried C. wilmsii on de novotranscription and translation during and after rehydration was determined by examining quantum efficiency, changes in photosynthetic pigments and subcellular organization of excised leaves with rehydration in water and using the metabolic inhibitors, distamycin A and cycloheximide. Slowly dried C. wilmsii required no new transcription or translation during rehydration in order to recover. With rapid dehydration, cells showed ultrastructural damage, which indicated that at least some protective mechanisms were affected (as evidenced by a reduced accumulation of sucrose). C. wilmsii was able to limit the damage and recover upon rehydration in water, but rapidly dried plants did not survive if mRNA or protein synthesis was inhibited by distamycin A or cycloheximide, respectively. This demonstrates an induction of repair mechanisms during rehydration, which enables recovery from rapid drying. Thus, although C. wilmsii does rely almost entirely on protection during natural drying, it apparently has the ability to repair if protection is inadequate and damage is incurred. en_ZA
dc.language eng en_ZA
dc.publisher Oxford University Press en_ZA
dc.source Journal of Experimental Botany en_ZA
dc.source.uri https://jxb.oxfordjournals.org/content/by/year
dc.subject.other Craterostigma wilmsii
dc.subject.other Drying rate
dc.subject.other Homoiochlorophyllous
dc.subject.other Metabolic inhibitors
dc.subject.other Modified desiccation
dc.title Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair en_ZA
dc.type Journal Article en_ZA
dc.date.updated 2016-08-01T10:50:19Z
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Cooper, K., & Farrant, J. M. (2002). Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair. <i>Journal of Experimental Botany</i>, http://hdl.handle.net/11427/21124 en_ZA
dc.identifier.chicagocitation Cooper, Keren, and Jill M Farrant "Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair." <i>Journal of Experimental Botany</i> (2002) http://hdl.handle.net/11427/21124 en_ZA
dc.identifier.vancouvercitation Cooper K, Farrant JM. Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair. Journal of Experimental Botany. 2002; http://hdl.handle.net/11427/21124. en_ZA
dc.identifier.ris TY - Journal Article AU - Cooper, Keren AU - Farrant, Jill M AB - Craterostigma wilmsii Engl. (homoiochlorophyllous) is a resurrection species that is thought to rely primarily on the protection of cellular components during drying to survive desiccation. The time taken for this protection to be instituted is thought to preclude recovery after rapid drying. Thus the response of C. wilmsii plants to rapid dehydration was investigated. The effect of rapid drying on sucrose accumulation was determined and the cellular ultrastructure was investigated during natural (slow) and rapid dehydration and on subsequent rehydration. The dependence of naturally and rapidly dried C. wilmsii on de novotranscription and translation during and after rehydration was determined by examining quantum efficiency, changes in photosynthetic pigments and subcellular organization of excised leaves with rehydration in water and using the metabolic inhibitors, distamycin A and cycloheximide. Slowly dried C. wilmsii required no new transcription or translation during rehydration in order to recover. With rapid dehydration, cells showed ultrastructural damage, which indicated that at least some protective mechanisms were affected (as evidenced by a reduced accumulation of sucrose). C. wilmsii was able to limit the damage and recover upon rehydration in water, but rapidly dried plants did not survive if mRNA or protein synthesis was inhibited by distamycin A or cycloheximide, respectively. This demonstrates an induction of repair mechanisms during rehydration, which enables recovery from rapid drying. Thus, although C. wilmsii does rely almost entirely on protection during natural drying, it apparently has the ability to repair if protection is inadequate and damage is incurred. DA - 2002 DB - OpenUCT DP - University of Cape Town J1 - Journal of Experimental Botany LK - https://open.uct.ac.za PB - University of Cape Town PY - 2002 SM - 0022-0957 T1 - Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair TI - Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection versus repair UR - http://hdl.handle.net/11427/21124 ER - en_ZA


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