Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration.

 

Show simple item record

dc.contributor.author Oyekola, Oluwaseun O
dc.contributor.author van Hille, Robert P
dc.contributor.author Harrison, Susan T L
dc.date.accessioned 2016-07-26T10:56:32Z
dc.date.available 2016-07-26T10:56:32Z
dc.date.issued 2010
dc.identifier http://dx.doi.org/10.1016/j.ces.2010.05.014
dc.identifier.citation Oyekola, O. O., van Hille, R. P., & Harrison, S. T. (2010). Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: effect of sulphate concentration. Chemical Engineering Science, 65(16), 4771-4781. en_ZA
dc.identifier.issn 0009-2509 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20760
dc.identifier.uri http://www.sciencedirect.com/science/article/pii/S0009250910003040
dc.description.abstract This study investigated the effect of feed sulphate concentration on the kinetics of anaerobic sulphate reduction by a mixed SRB culture, using lactate as the sole carbon source and electron donor. Chemostat cultures were operated across a range of residence times (0.5–5 d) and feed sulphate concentrations (1.0–10.0 g l1 ). Similar phenomena were observed at feed sulphate concentrations of 1.0 and 10.0 g l1 with the volumetric sulphate reduction rate increasing linearly with increasing volumetric sulphate loading rate. These reactors were characterised by higher specific volumetric sulphate reduction rates with maximum values of 0.24 and 0.20 g h1 g1 . Contrastingly, the reactors fed with sulphate concentrations of 2.5 and 5.0 g l1 showed distinctly different trends in which the volumetric sulphate reduction rate passed through a maximum at the dilution rates of 0.014 and 0.021 h1 , respectively, followed by a decline with further increase in sulphate loading rate. The maximum specific volumetric sulphate reduction rates observed were 2–6-fold lower than those observed at 1.0 and 10.0 g l1 feed sulphate concentrations. Profiles of specific volumetric sulphate reduction rate and biomass concentration suggested a shift in lactate utilisation from oxidation to fermentation at high dilution rates, implying a change in the dominant components of the microbial consortium. The data suggest that population structure was influenced by lactate affinity and dissolved sulphide concentration. The trends observed were attributed to the greater ability of lactate oxidisers to scavenge lactate under limiting concentrations of the substrate and their greater resilience to dissolved sulphide species in comparison to lactate fermenters. en_ZA
dc.language eng en_ZA
dc.publisher Elsevier en_ZA
dc.source Chemical Engineering Science en_ZA
dc.source.uri http://www.journals.elsevier.com/chemical-engineering-science/
dc.subject.other Acid mine drainage
dc.subject.other Bioprocessing
dc.subject.other Bioreactors
dc.subject.other Environment
dc.subject.other Kinetics
dc.subject.other Sulphate reducing bacteria
dc.title Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration. en_ZA
dc.type Journal Article en_ZA
dc.date.updated 2016-07-26T10:55:34Z
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Oyekola, O. O., van Hille, R. P., & Harrison, S. T. L. (2010). Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration. <i>Chemical Engineering Science</i>, http://hdl.handle.net/11427/20760 en_ZA
dc.identifier.chicagocitation Oyekola, Oluwaseun O, Robert P van Hille, and Susan T L Harrison "Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration." <i>Chemical Engineering Science</i> (2010) http://hdl.handle.net/11427/20760 en_ZA
dc.identifier.vancouvercitation Oyekola OO, van Hille RP, Harrison STL. Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration. Chemical Engineering Science. 2010; http://hdl.handle.net/11427/20760. en_ZA
dc.identifier.ris TY - Journal Article AU - Oyekola, Oluwaseun O AU - van Hille, Robert P AU - Harrison, Susan T L AB - This study investigated the effect of feed sulphate concentration on the kinetics of anaerobic sulphate reduction by a mixed SRB culture, using lactate as the sole carbon source and electron donor. Chemostat cultures were operated across a range of residence times (0.5–5 d) and feed sulphate concentrations (1.0–10.0 g l1 ). Similar phenomena were observed at feed sulphate concentrations of 1.0 and 10.0 g l1 with the volumetric sulphate reduction rate increasing linearly with increasing volumetric sulphate loading rate. These reactors were characterised by higher specific volumetric sulphate reduction rates with maximum values of 0.24 and 0.20 g h1 g1 . Contrastingly, the reactors fed with sulphate concentrations of 2.5 and 5.0 g l1 showed distinctly different trends in which the volumetric sulphate reduction rate passed through a maximum at the dilution rates of 0.014 and 0.021 h1 , respectively, followed by a decline with further increase in sulphate loading rate. The maximum specific volumetric sulphate reduction rates observed were 2–6-fold lower than those observed at 1.0 and 10.0 g l1 feed sulphate concentrations. Profiles of specific volumetric sulphate reduction rate and biomass concentration suggested a shift in lactate utilisation from oxidation to fermentation at high dilution rates, implying a change in the dominant components of the microbial consortium. The data suggest that population structure was influenced by lactate affinity and dissolved sulphide concentration. The trends observed were attributed to the greater ability of lactate oxidisers to scavenge lactate under limiting concentrations of the substrate and their greater resilience to dissolved sulphide species in comparison to lactate fermenters. DA - 2010 DB - OpenUCT DP - University of Cape Town J1 - Chemical Engineering Science LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 SM - 0009-2509 T1 - Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration TI - Kinetic analysis of biological sulphate reduction using lactate as carbon source and electron donor: Effect of sulphate concentration UR - http://hdl.handle.net/11427/20760 ER - en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record