Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant.

 

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dc.contributor.author Owor, Betty E
dc.contributor.author Martin, Darren P
dc.contributor.author Shepherd, Dionne N
dc.contributor.author Edema, Richard
dc.contributor.author Monjane, Ade´rito L
dc.contributor.author Rybicki, Edward P
dc.contributor.author Thomson, Jennifer A
dc.contributor.author Vasani, Arvind
dc.date.accessioned 2016-07-21T13:11:36Z
dc.date.available 2016-07-21T13:11:36Z
dc.date.issued 2007
dc.identifier http://dx.doi.org/10.1099/vir.0.83144-0
dc.identifier.citation Owor, B. E., Martin, D. P., Shepherd, D. N., Edema, R., Monjane, A. L., Rybicki, E. P., ... & Varsani, A. (2007). Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. Journal of general virology, 88(11), 3154-3165. en_ZA
dc.identifier.issn 0022-1317 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20571
dc.identifier.uri http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83144-0#tab2
dc.description.abstract Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants – MSV subtypes A1–A6 – causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR–restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for .60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species. en_ZA
dc.language eng en_ZA
dc.publisher Microbiology Society en_ZA
dc.source Journal of General Virology en_ZA
dc.source.uri http://jgv.microbiologyresearch.org/content/journal/jgv
dc.subject.other Genetic analysis
dc.subject.other maize streak virus
dc.subject.other Uganda
dc.subject.other recombinant variant
dc.title Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. en_ZA
dc.type Journal Article en_ZA
dc.date.updated 2016-07-21T13:10:21Z
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Owor, B. E., Martin, D. P., Shepherd, D. N., Edema, R., , Rybicki, E. P., ... Vasani, A. (2007). Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. <i>Journal of General Virology</i>, http://hdl.handle.net/11427/20571 en_ZA
dc.identifier.chicagocitation Owor, Betty E, Darren P Martin, Dionne N Shepherd, Richard Edema, , Edward P Rybicki, Jennifer A Thomson, and Arvind Vasani "Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant." <i>Journal of General Virology</i> (2007) http://hdl.handle.net/11427/20571 en_ZA
dc.identifier.vancouvercitation Owor BE, Martin DP, Shepherd DN, Edema R, , Rybicki EP, et al. Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. Journal of General Virology. 2007; http://hdl.handle.net/11427/20571. en_ZA
dc.identifier.ris TY - Journal Article AU - Owor, Betty E AU - Martin, Darren P AU - Shepherd, Dionne N AU - Edema, Richard AU - Monjane, Ade´rito L AU - Rybicki, Edward P AU - Thomson, Jennifer A AU - Vasani, Arvind AB - Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants – MSV subtypes A1–A6 – causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR–restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for .60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species. DA - 2007 DB - OpenUCT DP - University of Cape Town J1 - Journal of General Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 SM - 0022-1317 T1 - Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant TI - Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant UR - http://hdl.handle.net/11427/20571 ER - en_ZA


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