An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2

 

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dc.contributor.advisor Rawlings, Doug en_ZA
dc.contributor.author Smith, Anthony S G en_ZA
dc.date.accessioned 2016-07-19T14:20:52Z
dc.date.available 2016-07-19T14:20:52Z
dc.date.issued 1997 en_ZA
dc.identifier.citation Smith, A. 1997. An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20455
dc.description.abstract The broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2 is stably inherited over many generations despite a low copy number. The pas genes which lie between the repB primase and the repA helicase encode a proteic plasmid stabilisation system and are capable of stabilising the unstable heterologous R1 replicon present on p0U82. The deletion of the pas genes has been shown not to change the copy number of mutant plasmids. This suggested that the pas genes are not involved in replication and function as a stabilisation cassette. The pasA gene encodes an antidote, the pasB gene a toxin which exerts a bacteriocidal effect in an E. coli host and the pasC gene a protein which moderates the toxic effect of PasB. The PasC is unique in proteic plasmid stabilisation systems and reduces PasB toxicity only in the presence of PasA. PasA is able to repress the pas promoter and the addition of PasB increases this repression. PasC has been shown not to effect the pas promoter by itself. In the presence of PasA and PasB, PasC reduces the ability of PasA and PasB to repress the pas promoter. PasC is thought to stabilise the interaction of PasA and PasB and in doing so reduces their ability to function as repressors of the pas operon. The IncQ plasmid RSF1010 which has similarity to pTF-FC2 has two genes in a position analogous to the pasA, pasB and pasC genes. These genes have been found to be unable to function as a plasmid stabilisation system. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Molecular and Cell Biology en_ZA
dc.title An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2 en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Smith, A. S. G. (1997). <i>An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/20455 en_ZA
dc.identifier.chicagocitation Smith, Anthony S G. <i>"An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997. http://hdl.handle.net/11427/20455 en_ZA
dc.identifier.vancouvercitation Smith ASG. An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20455 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Smith, Anthony S G AB - The broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2 is stably inherited over many generations despite a low copy number. The pas genes which lie between the repB primase and the repA helicase encode a proteic plasmid stabilisation system and are capable of stabilising the unstable heterologous R1 replicon present on p0U82. The deletion of the pas genes has been shown not to change the copy number of mutant plasmids. This suggested that the pas genes are not involved in replication and function as a stabilisation cassette. The pasA gene encodes an antidote, the pasB gene a toxin which exerts a bacteriocidal effect in an E. coli host and the pasC gene a protein which moderates the toxic effect of PasB. The PasC is unique in proteic plasmid stabilisation systems and reduces PasB toxicity only in the presence of PasA. PasA is able to repress the pas promoter and the addition of PasB increases this repression. PasC has been shown not to effect the pas promoter by itself. In the presence of PasA and PasB, PasC reduces the ability of PasA and PasB to repress the pas promoter. PasC is thought to stabilise the interaction of PasA and PasB and in doing so reduces their ability to function as repressors of the pas operon. The IncQ plasmid RSF1010 which has similarity to pTF-FC2 has two genes in a position analogous to the pasA, pasB and pasC genes. These genes have been found to be unable to function as a plasmid stabilisation system. DA - 1997 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 T1 - An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2 TI - An investigation into the partition functions of the broad-host-range Plasmid pTF-FC2 UR - http://hdl.handle.net/11427/20455 ER - en_ZA


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