Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis

 

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dc.contributor.advisor Dheda, Keertan en_ZA
dc.contributor.author Fanampe, Boitumelo Louisa en_ZA
dc.date.accessioned 2016-07-08T10:43:27Z
dc.date.available 2016-07-08T10:43:27Z
dc.date.issued 2015 en_ZA
dc.identifier.citation Fanampe, B. 2015. Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20263
dc.description.abstract Tuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamma (IFN-γ) has been identified as a promising biomarker for the diagnosis of EPTB. The development of rapid and accurate point-of-care (POC) diagnostic technologies becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances even superior, to those of antibodies; in addition to their relatively low cost and simple method of production they have the potential to reduce assay turnaround time. The aim of this thesis was to develop aptamers to IFN-γ, a biomarker specific for EPTB, thus facilitating the development of aptamer-based POC tests for the diagnosis of EPTB. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Medicine en_ZA
dc.title Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis en_ZA
dc.type Doctoral Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Department of Medicine en_ZA
dc.type.qualificationlevel Doctoral
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Fanampe, B. L. (2015). <i>Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/20263 en_ZA
dc.identifier.chicagocitation Fanampe, Boitumelo Louisa. <i>"Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2015. http://hdl.handle.net/11427/20263 en_ZA
dc.identifier.vancouvercitation Fanampe BL. Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20263 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Fanampe, Boitumelo Louisa AB - Tuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamma (IFN-γ) has been identified as a promising biomarker for the diagnosis of EPTB. The development of rapid and accurate point-of-care (POC) diagnostic technologies becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances even superior, to those of antibodies; in addition to their relatively low cost and simple method of production they have the potential to reduce assay turnaround time. The aim of this thesis was to develop aptamers to IFN-γ, a biomarker specific for EPTB, thus facilitating the development of aptamer-based POC tests for the diagnosis of EPTB. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis TI - Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis UR - http://hdl.handle.net/11427/20263 ER - en_ZA


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