The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province

 

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dc.contributor.advisor Heyns, Marise en_ZA
dc.contributor.advisor Davies, Bronwen en_ZA
dc.contributor.author Visser, Hartwig en_ZA
dc.date.accessioned 2016-07-07T09:51:26Z
dc.date.available 2016-07-07T09:51:26Z
dc.date.issued 2016 en_ZA
dc.identifier.citation Visser, H. 2016. The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20247
dc.description.abstract Forensic entomologists rely on insect development and successional data to estimate the post-mortem interval (PMI). Ante-mortem drug use prior to an individual's death may result in drug transmission to feeding insects and subsequent alteration of their development, thereby altering PMI estimates. This study investigated the influence of Ritalin (methylphenidate hydrochloride) (MPH) on the development of Chrysomya chloropyga. C. chloropyga larvae were reared on pig liver treated with MPH and exhibited a trend of expedited larval development and prolonged pupal development. Conservatively, the results suggest that MPH may expedite the larval stage by up to 17 hours and prolong the pupal stage by up to 16 hours. These preliminary findings suggest that, at the concentration investigated, MPH may alter the duration of C. chloropyga developmental stages, and consequently PMI estimates if MPH is not detected or its effects not considered. Furthermore, MPH was detected in both frozen and ethanol preserved specimens. MPH could still be detected from treated larvae, after 3.5 days incubation at ~30°C. This may suggest an improved stability of MPH in insects. Moreover, detection of MPH from ethanol preserved specimens suggests the qualitative toxicological utility of specimens maintained in this preservation liquid, despite stability and self-extraction concerns. These are the first entomotoxicological data on MPH generated for blow flies local to the Western Cape, South Africa. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Biomedical Forensic Science en_ZA
dc.title The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of Forensic Medicine and Toxicology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MPhil en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Visser, H. (2016). <i>The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology. Retrieved from http://hdl.handle.net/11427/20247 en_ZA
dc.identifier.chicagocitation Visser, Hartwig. <i>"The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016. http://hdl.handle.net/11427/20247 en_ZA
dc.identifier.vancouvercitation Visser H. The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Forensic Medicine and Toxicology, 2016 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20247 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Visser, Hartwig AB - Forensic entomologists rely on insect development and successional data to estimate the post-mortem interval (PMI). Ante-mortem drug use prior to an individual's death may result in drug transmission to feeding insects and subsequent alteration of their development, thereby altering PMI estimates. This study investigated the influence of Ritalin (methylphenidate hydrochloride) (MPH) on the development of Chrysomya chloropyga. C. chloropyga larvae were reared on pig liver treated with MPH and exhibited a trend of expedited larval development and prolonged pupal development. Conservatively, the results suggest that MPH may expedite the larval stage by up to 17 hours and prolong the pupal stage by up to 16 hours. These preliminary findings suggest that, at the concentration investigated, MPH may alter the duration of C. chloropyga developmental stages, and consequently PMI estimates if MPH is not detected or its effects not considered. Furthermore, MPH was detected in both frozen and ethanol preserved specimens. MPH could still be detected from treated larvae, after 3.5 days incubation at ~30°C. This may suggest an improved stability of MPH in insects. Moreover, detection of MPH from ethanol preserved specimens suggests the qualitative toxicological utility of specimens maintained in this preservation liquid, despite stability and self-extraction concerns. These are the first entomotoxicological data on MPH generated for blow flies local to the Western Cape, South Africa. DA - 2016 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 T1 - The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province TI - The influence of Methylphenidate on the development of the forensically significant blow fly Chrysomya chloropyga (Diptera: Calliphoridae) in the Western Cape Province UR - http://hdl.handle.net/11427/20247 ER - en_ZA


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