A study of the molecular variability of some South African isolates of tobacco necrosis virus

 

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dc.contributor.advisor Von Wechmar, M Barbara en_ZA
dc.contributor.advisor Purves, M en_ZA
dc.contributor.author Freeborough, Michael-John en_ZA
dc.date.accessioned 2016-07-04T08:42:26Z
dc.date.available 2016-07-04T08:42:26Z
dc.date.issued 1996 en_ZA
dc.identifier.citation Freeborough, M. 1996. A study of the molecular variability of some South African isolates of tobacco necrosis virus. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20192
dc.description Bibliography: pages 109-118. en_ZA
dc.description.abstract The relationship of various tobacco necrosis viruses, isolated from various crops and other sources in South Africa was determined. An isolate from avocado was chosen for partial characterisation to confirm that it was a member of the Necrovirus group of plant viruses. TNV was detected in potatoes that exhibited abnormal necrotic foliar symptoms and a D-type TNV was isolated and identified from the Up-to-Date potato variety. Immunoelectroblotting assay grouped the TNV isolates studied into two serotypes (A and D). This result was confirmed by NA hybridization with probes derived to the coat protein of an A- (TNVWheat) and a D-type (TNV-Papaya green lesion) isolate. RT-PCR with A and D specific primers did not amplify the coat protein of three A- and D-type TNV isolates which appears to indicate that the detection by PCR with specific primers is too selective to be used for a general test, unless degenerate primers to a more conserved region of the coat protein gene are used. Sequence analysis of the coat protein gene was used to determine the phylogenetic relationship amongst nine TNV isolates examined and also by comparison to three isolates for which the coat protein gene had already been sequenced. Sequence analysis showed high degree of homology amongst the A-type isolates and the D-type isolates, with approximately 45 % homology between the two TNV types. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Molecular and Cell Biology en_ZA
dc.title A study of the molecular variability of some South African isolates of tobacco necrosis virus en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image


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