Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36

 

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dc.contributor.advisor Harrison, STL en_ZA
dc.contributor.advisor Fenner, Caryn en_ZA
dc.contributor.author Mrisho, Latifa Mbwana en_ZA
dc.date.accessioned 2016-06-22T08:57:55Z
dc.date.available 2016-06-22T08:57:55Z
dc.date.issued 2015 en_ZA
dc.identifier.citation Mrisho, L. 2015. Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/20089
dc.description.abstract Amylases are hydrolytic enzymes that cause the breakdown of starch and related polysaccharides to simple sugars. Amylases are applied in brewing, food, detergent and textile industries. Most commercial amylases are derived from fungi or bacteria. Bacterial amylases are desired for commercial use, due to their thermo-stability and faster production rates. Bacteria of the genus, Bacillus, are considered to be a good source of extracellular proteins because they have high growth rates and have a naturally high capacity for secretion of extracellular proteins. Bacillus halodurans Alk36 is an alkaliphilic, thermotolerant isolate that can grow over a wide pH and temperature range. Preliminary studies have shown that B. halodurans Alk36 can grown in EnBase® medium (at pH 8.5) containing starch as the carbon source, without the addition of a commercial amylase. The ability to grow on starch, in the absence of an external amylase, indicated that this strain produces endogenous alkaliphilic amylases, which may be exploited for a number of industrial applications. In the present study, the physiological and biochemical characterisation of B. halodurans Alk36 and its endogenous amylases were investigated. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Chemical Engineering en_ZA
dc.title Production and characterization of alkaliphilic amylases from Bacillus halodurans Alk36 en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Engineering & the Built Environment en_ZA
dc.publisher.department Department of Chemical Engineering en_ZA
dc.type.qualificationlevel Masters en_ZA
dc.type.qualificationname MSc (Eng) en_ZA
uct.type.filetype Text
uct.type.filetype Image


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