The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli

 

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dc.contributor.advisor Abratt, Valerie Rose en_ZA
dc.contributor.author Samsodien, Anwar en_ZA
dc.date.accessioned 2016-05-16T11:58:40Z
dc.date.available 2016-05-16T11:58:40Z
dc.date.issued 1997 en_ZA
dc.identifier.citation Samsodien, A. 1997. The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/19682
dc.description Bibliography: pages 119-137. en_ZA
dc.description.abstract Butyrivibrio fibrisolvens HI 7C is an important anaerobic bacterium which occurs in the rumen of most ruminants. A key factor affecting the growth of this bacterium is the availability of nitrogen sources, particularly in the form of ammonia. The aims of this study were to attempt the isolation of a gene/genes involved in the regulation of the B.fibrisolvens type III glutamine synthetase (GS), which is a key enzyme involved in ammonia assimilation in rumen bacteria. An existing B. fibrisolvens gene bank, as well as the B. fibrisolvens glnA gene cloned onto a low 11 copy number plasmid, were used to generate a heterologous, Escherichia coli- based, two plasmid, in trans system. This system was used to isolate an E. coli clone which showed increased GS activity levels (2.5-fold) and a retarded growth rate phenotype in complete media. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Molecular and Cell Biology en_ZA
dc.title The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli en_ZA
dc.type Master Thesis
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Science en_ZA
dc.publisher.department Department of Molecular and Cell Biology en_ZA
dc.type.qualificationlevel Masters
dc.type.qualificationname MSc en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Samsodien, A. (1997). <i>The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/19682 en_ZA
dc.identifier.chicagocitation Samsodien, Anwar. <i>"The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997. http://hdl.handle.net/11427/19682 en_ZA
dc.identifier.vancouvercitation Samsodien A. The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/19682 en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Samsodien, Anwar AB - Butyrivibrio fibrisolvens HI 7C is an important anaerobic bacterium which occurs in the rumen of most ruminants. A key factor affecting the growth of this bacterium is the availability of nitrogen sources, particularly in the form of ammonia. The aims of this study were to attempt the isolation of a gene/genes involved in the regulation of the B.fibrisolvens type III glutamine synthetase (GS), which is a key enzyme involved in ammonia assimilation in rumen bacteria. An existing B. fibrisolvens gene bank, as well as the B. fibrisolvens glnA gene cloned onto a low 11 copy number plasmid, were used to generate a heterologous, Escherichia coli- based, two plasmid, in trans system. This system was used to isolate an E. coli clone which showed increased GS activity levels (2.5-fold) and a retarded growth rate phenotype in complete media. DA - 1997 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 T1 - The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli TI - The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli UR - http://hdl.handle.net/11427/19682 ER - en_ZA


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