Real-time investigation of tuberculosis transmission: developing the Respiratory Aerosol Sampling Chamber (RASC)

 

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dc.contributor.author Wood, Robin en_ZA
dc.contributor.author Morrow, Carl en_ZA
dc.contributor.author III, Clifton E Barry en_ZA
dc.contributor.author Bryden, Wayne A en_ZA
dc.contributor.author Call, Charles J en_ZA
dc.contributor.author Hickey, Anthony J en_ZA
dc.contributor.author Rodes, Charles E en_ZA
dc.contributor.author Scriba, Thomas J en_ZA
dc.contributor.author Blackburn, Jonathan en_ZA
dc.contributor.author Issarow, Chacha en_ZA
dc.contributor.author Mulder, Nicola en_ZA
dc.contributor.author Woodward, Jeremy en_ZA
dc.contributor.author Moosa, Atica en_ZA
dc.contributor.author Singh, Vinayak en_ZA
dc.contributor.author Mizrahi, Valerie en_ZA
dc.contributor.author Warner, Digby F en_ZA
dc.date.accessioned 2016-03-08T10:54:14Z
dc.date.available 2016-03-08T10:54:14Z
dc.date.issued 2016 en_ZA
dc.identifier.citation Wood, R., Morrow, C., Barry III, C. E., Bryden, W. A., Call, C. J., Hickey, A. J., ... & Mulder, N. (2016). Real-time investigation of tuberculosis transmission: developing the Respiratory Aerosol Sampling Chamber (RASC). PloS one, 11(1). doi:10.1371/journal.pone.0146658 en_ZA
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0146658 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/17570
dc.description.abstract Knowledge of the airborne nature of respiratory disease transmission owes much to the pioneering experiments of Wells and Riley over half a century ago. However, the mechanical, physiological, and immunopathological processes which drive the production of infectious aerosols by a diseased host remain poorly understood. Similarly, very little is known about the specific physiological, metabolic and morphological adaptations which enable pathogens such as Mycobacterium tuberculosis ( Mtb ) to exit the infected host, survive exposure to the external environment during airborne carriage, and adopt a form that is able to enter the respiratory tract of a new host, avoiding innate immune and physical defenses to establish a nascent infection. As a first step towards addressing these fundamental knowledge gaps which are central to any efforts to interrupt disease transmission, we developed and characterized a small personal clean room comprising an array of sampling devices which enable isolation and representative sampling of airborne particles and organic matter from tuberculosis (TB) patients. The complete unit, termed the Respiratory Aerosol Sampling Chamber (RASC), is instrumented to provide real-time information about the particulate output of a single patient, and to capture samples via a suite of particulate impingers, impactors and filters. Applying the RASC in a clinical setting, we demonstrate that a combination of molecular and microbiological assays, as well as imaging by fluorescence and scanning electron microscopy, can be applied to investigate the identity, viability, and morphology of isolated aerosolized particles. Importantly, from a preliminary panel of active TB patients, we observed the real-time production of large numbers of airborne particles including Mtb , as confirmed by microbiological culture and polymerase chain reaction (PCR) genotyping. Moreover, direct imaging of captured samples revealed the presence of multiple rod-like Mtb organisms whose physical dimensions suggested the capacity for travel deep into the alveolar spaces of the human lung. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Aerosols en_ZA
dc.subject.other Mycobacterium tuberculosis en_ZA
dc.subject.other Particle size en_ZA
dc.subject.other Tuberculosis en_ZA
dc.subject.other Aerodynamics en_ZA
dc.subject.other Respiratory infections en_ZA
dc.subject.other Carbon dioxide en_ZA
dc.subject.other Scanning electron microscopy en_ZA
dc.title Real-time investigation of tuberculosis transmission: developing the Respiratory Aerosol Sampling Chamber (RASC) en_ZA
dc.type Journal Article en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Institute of Infectious Disease and Molecular Medicine en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.