Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study

 

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dc.contributor.advisor Isaacson, Leon en_ZA
dc.contributor.author Saunders, James en_ZA
dc.date.accessioned 2016-02-29T11:55:35Z
dc.date.available 2016-02-29T11:55:35Z
dc.date.issued 1992 en_ZA
dc.identifier.citation Saunders, J. 1992. Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study. University of Cape Town. en_ZA
dc.identifier.uri http://hdl.handle.net/11427/17308
dc.description Includes bibliography. en_ZA
dc.description.abstract The presence of calcium (Ca) channels has been investigated in the apical membrane of various segments of rabbit renal tubules and in the basolateral membrane of human tubules, using the patch clamp technique. The rabbit tubule segments comprised proximal straight tubules (PST), thick ascending limbs (TAL), distal convoluted tubules (OCT), and cortical collecting ducts (CCD). The human tubule segments could not be identified, but were probably of proximal origin. The luminal surfaces of the individual tubule segments were accessed by perfusing the tubule and inserting the patch pipette through the open end or, more frequently, by tearing open the tubule to allow direct access by a patch electrode. Either Ba (90 mM) or Ca (70 mM) was used in the pipettes. Where possible, channel activity was sought in voltage clamped (30 to -60 m V) excised patches. The data were digitized at l kHz, and filtered (200- 500 Hz) by a six-pole Bessel filter. en_ZA
dc.language.iso eng en_ZA
dc.subject.other Physiology en_ZA
dc.title Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study en_ZA
dc.type Thesis / Dissertation en_ZA
uct.type.publication Research en_ZA
uct.type.resource Thesis en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department MRC/UCT RU for Exercise and Sport Medicine en_ZA
dc.type.qualificationlevel Doctoral en_ZA
dc.type.qualificationname PhD en_ZA
uct.type.filetype Text
uct.type.filetype Image


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