A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults

 

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dc.contributor.author Ritz, Nicole en_ZA
dc.contributor.author Strach, Madeleine en_ZA
dc.contributor.author Yau, Carmen en_ZA
dc.contributor.author Dutta, Binita en_ZA
dc.contributor.author Tebruegge, Marc en_ZA
dc.contributor.author Connell, Tom G en_ZA
dc.contributor.author Hanekom, Willem A en_ZA
dc.contributor.author Britton, Warwick J en_ZA
dc.contributor.author Robins-Browne, Roy en_ZA
dc.contributor.author Curtis, Nigel en_ZA
dc.date.accessioned 2016-01-11T06:52:11Z
dc.date.available 2016-01-11T06:52:11Z
dc.date.issued 2012 en_ZA
dc.identifier.citation Ritz, N., Strach, M., Yau, C., Dutta, B., Tebruegge, M., Connell, T. G., ... & Curtis, N. (2012). A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults. PloS one, 7(7), e37535. doi:10.1371/journal.pone.0037535 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/16258
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0037535
dc.description.abstract BCG vaccine is one of the most commonly-administered vaccines worldwide. Studies suggest the protective efficacy of BCG against TB is better for children than for adults. One potential explanation is that BCG induces a better protective immune response in children. Twenty six children and adults were immunised with BCG. The proportion of Th1-cytokine-producing mycobacterial-specific T cells, and the concentrations of secreted cytokines, were measured before and 10 weeks after BCG immunisation. A significant increase in the proportion of mycobacterial-specific cytokine-producing T cells was observed in both age groups. After BCG immunisation, children and adults had comparable proportions of mycobacterial-specific polyfunctional CD4 T cells when measured relative to the total number of CD4 T cells. However, relative to the subset of Th-1-cytokine-producing CD4 T cells, the proportion of polyfunctional cells was greater in children. Concentrations of secreted cytokines were comparable in children and adults. These findings suggest that the mycobacterial-specific cell-mediated immune response induced by BCG immunisation in children and adults is similar. The implication of a shift to a more polyfunctional immune response within the Th1-cytokine-producing CD4 T cells in children is uncertain as this aspect of the immune response has not been assessed as a potential correlate of protection against TB. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other T cells en_ZA
dc.subject.other T helper cells en_ZA
dc.subject.other Cytokines en_ZA
dc.subject.other Immune response en_ZA
dc.subject.other Children en_ZA
dc.subject.other Adults en_ZA
dc.subject.other Cytotoxic T cells en_ZA
dc.subject.other Tuberculosis en_ZA
dc.title A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2012 Ritz et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department South African Tuberculosis Vaccine Initiative (SATVI) en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Ritz, N., Strach, M., Yau, C., Dutta, B., Tebruegge, M., Connell, T. G., ... Curtis, N. (2012). A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults. <i>PLoS One</i>, http://hdl.handle.net/11427/16258 en_ZA
dc.identifier.chicagocitation Ritz, Nicole, Madeleine Strach, Carmen Yau, Binita Dutta, Marc Tebruegge, Tom G Connell, Willem A Hanekom, Warwick J Britton, Roy Robins-Browne, and Nigel Curtis "A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults." <i>PLoS One</i> (2012) http://hdl.handle.net/11427/16258 en_ZA
dc.identifier.vancouvercitation Ritz N, Strach M, Yau C, Dutta B, Tebruegge M, Connell TG, et al. A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults. PLoS One. 2012; http://hdl.handle.net/11427/16258. en_ZA
dc.identifier.ris TY - Journal Article AU - Ritz, Nicole AU - Strach, Madeleine AU - Yau, Carmen AU - Dutta, Binita AU - Tebruegge, Marc AU - Connell, Tom G AU - Hanekom, Willem A AU - Britton, Warwick J AU - Robins-Browne, Roy AU - Curtis, Nigel AB - BCG vaccine is one of the most commonly-administered vaccines worldwide. Studies suggest the protective efficacy of BCG against TB is better for children than for adults. One potential explanation is that BCG induces a better protective immune response in children. Twenty six children and adults were immunised with BCG. The proportion of Th1-cytokine-producing mycobacterial-specific T cells, and the concentrations of secreted cytokines, were measured before and 10 weeks after BCG immunisation. A significant increase in the proportion of mycobacterial-specific cytokine-producing T cells was observed in both age groups. After BCG immunisation, children and adults had comparable proportions of mycobacterial-specific polyfunctional CD4 T cells when measured relative to the total number of CD4 T cells. However, relative to the subset of Th-1-cytokine-producing CD4 T cells, the proportion of polyfunctional cells was greater in children. Concentrations of secreted cytokines were comparable in children and adults. These findings suggest that the mycobacterial-specific cell-mediated immune response induced by BCG immunisation in children and adults is similar. The implication of a shift to a more polyfunctional immune response within the Th1-cytokine-producing CD4 T cells in children is uncertain as this aspect of the immune response has not been assessed as a potential correlate of protection against TB. DA - 2012 DB - OpenUCT DO - 10.1371/journal.pone.0037535 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults TI - A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guerin immunisation in children and adults UR - http://hdl.handle.net/11427/16258 ER - en_ZA


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