Seminal plasma enhances cervical adenocarcinoma cell proliferation and tumour growth in vivo

 

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dc.contributor.author Sutherland, Jason Robert en_ZA
dc.contributor.author Sales, Kurt J en_ZA
dc.contributor.author Jabbour, Henry N en_ZA
dc.contributor.author Katz, Arieh A en_ZA
dc.date.accessioned 2016-01-11T06:46:19Z
dc.date.available 2016-01-11T06:46:19Z
dc.date.issued 2012 en_ZA
dc.identifier.citation Sutherland, J. R., Sales, K. J., Jabbour, H. N., & Katz, A. A. (2012). Seminal plasma enhances cervical adenocarcinoma cell proliferation and tumour growth in vivo. PLoS One, 7(3), e33848. doi:10.1371/journal.pone.0033848 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/16220
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0033848
dc.description.abstract Cervical cancer is one of the leading causes of cancer-related death in women in sub-Saharan Africa. Extensive evidence has shown that cervical cancer and its precursor lesions are caused by Human papillomavirus (HPV) infection. Although the vast majority of HPV infections are naturally resolved, failure to eradicate infected cells has been shown to promote viral persistence and tumorigenesis. Furthermore, following neoplastic transformation, exposure of cervical epithelial cells to inflammatory mediators either directly or via the systemic circulation may enhance progression of the disease. It is well recognised that seminal plasma contains an abundance of inflammatory mediators, which are identified as regulators of tumour growth. Here we investigated the role of seminal plasma in regulating neoplastic cervical epithelial cell growth and tumorigenesis. Using HeLa cervical adenocarcinoma cells, we found that seminal plasma (SP) induced the expression of the inflammatory enzymes, prostaglandin endoperoxide synthase (PTGS1 and PTGS2), cytokines interleukin (IL) -6, and -11 and vascular endothelial growth factor-A(VEGF-A). To investigate the role of SP on tumour cell growth in vivo, we xenografted HeLa cells subcutaneously into the dorsal flank of nude mice. Intra-peritoneal administration of SP rapidly and significantly enhanced the tumour growth rate and size of HeLa cell xenografts in nude mice. As observed in vitro, we found that SP induced expression of inflammatory PTGS enzymes, cytokines and VEGF-A in vivo. Furthermore we found that SP enhances blood vessel size in HeLa cell xenografts. Finally we show that SP-induced cytokine production, VEGF-A expression and cell proliferation are mediated via the induction of the inflammatory PTGS pathway. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other HeLa cells en_ZA
dc.subject.other Inflammation en_ZA
dc.subject.other Cancer treatment en_ZA
dc.subject.other Cytokines en_ZA
dc.subject.other Gene expression en_ZA
dc.subject.other Blood plasma en_ZA
dc.subject.other Mouse models en_ZA
dc.subject.other Cervical cancer en_ZA
dc.title Seminal plasma enhances cervical adenocarcinoma cell proliferation and tumour growth in vivo en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2012 Sutherland et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department MRC/UCT Receptor Biology Research Group en_ZA
uct.type.filetype Text
uct.type.filetype Image


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.