Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children

 

Show simple item record

dc.contributor.author Dube, Felix S en_ZA
dc.contributor.author Kaba, Mamadou en_ZA
dc.contributor.author Whittaker, Elizabeth en_ZA
dc.contributor.author Zar, Heather J en_ZA
dc.contributor.author Nicol, Mark P en_ZA
dc.date.accessioned 2015-12-28T06:51:42Z
dc.date.available 2015-12-28T06:51:42Z
dc.date.issued 2013 en_ZA
dc.identifier.citation Dube, F. S., Kaba, M., Whittaker, E., Zar, H. J., & Nicol, M. P. (2013). Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children. PloS one, 8(6), e68097. doi:10.1371/journal.pone.0068097 en_ZA
dc.identifier.uri http://hdl.handle.net/11427/16069
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0068097
dc.description.abstract BACKGROUND: A better understanding of the epidemiology of nasopharyngeal carriage of Streptococcus pneumoniae is important to assess the impact of vaccination and the pathogenesis of pneumococcal disease. We compared the recovery of S. pneumoniae from nylon flocked, Dacron and rayon swabs. METHODS: The recovery of S. pneumoniae from mocked specimens using flocked, Dacron and rayon swabs were compared by culture. The yield from paired nasopharyngeal (NP) samples obtained from healthy children sampled with flocked and Dacron swabs was also determined using culture and lytA -targeted real-time polymerase chain reaction (qPCR). RESULTS: Using mock specimen, the percentage recovery of S. pneumoniae ATCC 49619 (serotype 19F) strain from the flocked swabs was 100%, while it was 41% from Dacron swabs and 7% from rayon swabs. Similar results were observed for S. pneumoniae serotypes 1 and 5. S. pneumoniae was cultured from 18 of 42 (43%) paired NP samples from the healthy children (median age 8 [interquartile range (IQR) 5-16] months). The median number of colony-forming units (CFU) recovered from flocked swabs was two-fold higher (8.8×10 4 CFU/mL [IQR, 2.0×10 2 - 4.0×10 5 CFU/mL]) than Dacron swabs (3.7×10 4 CFU/mL [IQR, 4.0×10 2 -3.2×10 5 CFU/mL], p  = 0.17). Using lytA -targeted qPCR from paired NP samples, the median copy number of S. pneumoniae detected from flocked swabs was significantly higher than from Dacron swabs (3.0×10 5 genome copies/mL [IQR, 1.3×10 2 −1.8×10 6 ] vs. 9.3×10 4 genome copies/mL [IQR, 7.0×10 1 −1.1×10 6 ]; p  = 0.005). CONCLUSION: Flocked swabs released more S. pneumoniae compared to both Dacron and rayon swabs from mock specimens. Similarly, higher bacterial loads were detected by qPCR from flocked swabs compared with Dacron swabs from healthy children. en_ZA
dc.language.iso eng en_ZA
dc.publisher Public Library of Science en_ZA
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.rights.uri http://creativecommons.org/licenses/by/4.0 en_ZA
dc.source PLoS One en_ZA
dc.source.uri http://journals.plos.org/plosone en_ZA
dc.subject.other Children en_ZA
dc.subject.other Child health en_ZA
dc.subject.other Polymerase chain reaction en_ZA
dc.subject.other Pneumococcus en_ZA
dc.subject.other Nucleic acids en_ZA
dc.subject.other Pneumonia en_ZA
dc.subject.other DNA extraction en_ZA
dc.subject.other Health services research en_ZA
dc.title Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children en_ZA
dc.type Journal Article en_ZA
dc.rights.holder © 2013 Dube et al en_ZA
uct.type.publication Research en_ZA
uct.type.resource Article en_ZA
dc.publisher.institution University of Cape Town
dc.publisher.faculty Faculty of Health Sciences en_ZA
dc.publisher.department Division of Medical Biochemistry en_ZA
uct.type.filetype Text
uct.type.filetype Image
dc.identifier.apacitation Dube, F. S., Kaba, M., Whittaker, E., Zar, H. J., & Nicol, M. P. (2013). Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children. <i>PLoS One</i>, http://hdl.handle.net/11427/16069 en_ZA
dc.identifier.chicagocitation Dube, Felix S, Mamadou Kaba, Elizabeth Whittaker, Heather J Zar, and Mark P Nicol "Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children." <i>PLoS One</i> (2013) http://hdl.handle.net/11427/16069 en_ZA
dc.identifier.vancouvercitation Dube FS, Kaba M, Whittaker E, Zar HJ, Nicol MP. Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children. PLoS One. 2013; http://hdl.handle.net/11427/16069. en_ZA
dc.identifier.ris TY - Journal Article AU - Dube, Felix S AU - Kaba, Mamadou AU - Whittaker, Elizabeth AU - Zar, Heather J AU - Nicol, Mark P AB - BACKGROUND: A better understanding of the epidemiology of nasopharyngeal carriage of Streptococcus pneumoniae is important to assess the impact of vaccination and the pathogenesis of pneumococcal disease. We compared the recovery of S. pneumoniae from nylon flocked, Dacron and rayon swabs. METHODS: The recovery of S. pneumoniae from mocked specimens using flocked, Dacron and rayon swabs were compared by culture. The yield from paired nasopharyngeal (NP) samples obtained from healthy children sampled with flocked and Dacron swabs was also determined using culture and lytA -targeted real-time polymerase chain reaction (qPCR). RESULTS: Using mock specimen, the percentage recovery of S. pneumoniae ATCC 49619 (serotype 19F) strain from the flocked swabs was 100%, while it was 41% from Dacron swabs and 7% from rayon swabs. Similar results were observed for S. pneumoniae serotypes 1 and 5. S. pneumoniae was cultured from 18 of 42 (43%) paired NP samples from the healthy children (median age 8 [interquartile range (IQR) 5-16] months). The median number of colony-forming units (CFU) recovered from flocked swabs was two-fold higher (8.8×10 4 CFU/mL [IQR, 2.0×10 2 - 4.0×10 5 CFU/mL]) than Dacron swabs (3.7×10 4 CFU/mL [IQR, 4.0×10 2 -3.2×10 5 CFU/mL], p  = 0.17). Using lytA -targeted qPCR from paired NP samples, the median copy number of S. pneumoniae detected from flocked swabs was significantly higher than from Dacron swabs (3.0×10 5 genome copies/mL [IQR, 1.3×10 2 −1.8×10 6 ] vs. 9.3×10 4 genome copies/mL [IQR, 7.0×10 1 −1.1×10 6 ]; p  = 0.005). CONCLUSION: Flocked swabs released more S. pneumoniae compared to both Dacron and rayon swabs from mock specimens. Similarly, higher bacterial loads were detected by qPCR from flocked swabs compared with Dacron swabs from healthy children. DA - 2013 DB - OpenUCT DO - 10.1371/journal.pone.0068097 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2013 T1 - Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children TI - Detection of Streptococcus pneumoniae from different types of nasopharyngeal swabs in children UR - http://hdl.handle.net/11427/16069 ER - en_ZA


Files in this item

This item appears in the following Collection(s)

Show simple item record

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.